I am going to extract mt-DNA from hair and urine for my research purposes. I need a manual protocol for it not of the Standardized Kit that are available. It maybe in form of papers.
As cal is defined as the approximate amount of energy needed to raise the temperature of one gram of water by one degree Celsius at a pressure of one atmosphere. It would be nice if someone can...
09 October 2017 283 2 View
I have a problem, my plate and spacer are fused because of acrylamide and it's hard to remove I even broke one short plate. Our acrylamide is not good to use we confirmed that by an acrylamide...
05 June 2017 8,960 8 View
Hey there, can someone explain me the the first law and the second law of thermodynamics followed by the cell, the energy, heat and the pressure. The enthalpy and entropy and in such scenario and...
10 November 2016 1,475 1 View
Can someone please explain in detail with examples to clarify me the main difference between ligand, substrate and co-factors. I am curious about it. If there is no difference then why using the...
06 July 2016 4,617 5 View
Can someone please explain with good examples about regioselectivity and stereoselectivity? In general chemical bonding concept and it would be perfect if someone could explain with respect to an...
04 May 2016 5,730 1 View
I am reading an article which says " The UVC light induces the formation of covalent cross-links only at the sites of direct contact between protein and RNA".As UVC is used for disinfecting water,...
03 April 2016 5,113 1 View
I am extracting DNA from Saliva through Manual Protocol NOT with the Standardized Kits. Right at the end of the extraction when I get the DNA Pallet that need to be dissolved by adding de-ionized...
08 September 2014 2,927 29 View
I was thinking that to be a good Geneticist one should have the knowledge of: 01: Mathematics with calculus 02: Statistics 03: Physics Should one master the above three subjects or to what extent...
31 December 2013 9,338 3 View
I was reading an article, where I came across with " Thiolated DNA." How does a DTT chemical act to make a bond with the sulfur of Thiolated DNA? Please inform me about the structure of Thiolated...
11 December 2013 9,992 3 View
I am going to extract DNA from urine samples. Could anyone guide me, who has extracted DNA from urine manually not from the commercial kits? I am not getting any paper that would have proper...
11 December 2013 2,653 5 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View