I am going to extract mt-DNA from hair and urine for my research purposes. I need a manual protocol for it not of the Standardized Kit that are available. It maybe in form of papers.
As cal is defined as the approximate amount of energy needed to raise the temperature of one gram of water by one degree Celsius at a pressure of one atmosphere. It would be nice if someone can...
09 October 2017 208 2 View
I have a problem, my plate and spacer are fused because of acrylamide and it's hard to remove I even broke one short plate. Our acrylamide is not good to use we confirmed that by an acrylamide...
05 June 2017 8,890 8 View
Hey there, can someone explain me the the first law and the second law of thermodynamics followed by the cell, the energy, heat and the pressure. The enthalpy and entropy and in such scenario and...
10 November 2016 1,406 1 View
Can someone please explain in detail with examples to clarify me the main difference between ligand, substrate and co-factors. I am curious about it. If there is no difference then why using the...
06 July 2016 4,533 5 View
Can someone please explain with good examples about regioselectivity and stereoselectivity? In general chemical bonding concept and it would be perfect if someone could explain with respect to an...
04 May 2016 5,675 1 View
I am reading an article which says " The UVC light induces the formation of covalent cross-links only at the sites of direct contact between protein and RNA".As UVC is used for disinfecting water,...
03 April 2016 5,046 1 View
I am extracting DNA from Saliva through Manual Protocol NOT with the Standardized Kits. Right at the end of the extraction when I get the DNA Pallet that need to be dissolved by adding de-ionized...
08 September 2014 2,851 29 View
I was thinking that to be a good Geneticist one should have the knowledge of: 01: Mathematics with calculus 02: Statistics 03: Physics Should one master the above three subjects or to what extent...
31 December 2013 9,272 3 View
I was reading an article, where I came across with " Thiolated DNA." How does a DTT chemical act to make a bond with the sulfur of Thiolated DNA? Please inform me about the structure of Thiolated...
11 December 2013 9,921 3 View
I am going to extract DNA from urine samples. Could anyone guide me, who has extracted DNA from urine manually not from the commercial kits? I am not getting any paper that would have proper...
11 December 2013 2,570 5 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...
03 March 2021 8,796 3 View
I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...
03 March 2021 1,931 5 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View