How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View

No title

This phase is a ternary phase in Al-rich region of Al-Ni-Cr alloy system with a typical chemical composition of Al74Ni8Cr18.

06 September 2020 5,950 3 View

Please guide: Why my PCR lacks reproducibilty?

whenever i buy new primers (100pM) they work fine at first dilution (10pM) as well as first 3-4 polymerase chain reactions. But when i use same or new dilutions (10pM) after 15- 20 days at the...

20 July 2020 5,371 16 View

What can be possible reason when there is no reproducibilty in PCR which ones functioned?

When i run gradient PCR, it works well for 2-3 days, After that there is no amplification neither in same or other gradient ranges nor at temperature specified by first run. Is this issue with...

17 March 2020 1,643 2 View

Is it possible to see the redox peak in CV while did not observe plateaus in the charge-discharge curves?

Dear researchers I see clear redox peaks in CV, but i did not plateau in the charge-discharge curves. Does anyone know the reason?

04 February 2020 707 9 View

Has anyone ever experience for Aflatoxin determination in meat by HPLC?

Has anyone ever experience for Aflatoxin determination in meat by HPLC?

29 December 2019 389 4 View

What are the antenna design challenges for 5G handset uses,these days?

any suggestion on recently designed antennas for 5G handset uses.

08 November 2019 5,509 4 View

What is the concept of mass collaboration ?

collaboration meaning

13 June 2019 4,233 3 View

What is the equivalent conditions for strongly \pi regular rings?

hello im looking for a equivalent conditions for strpngly \pi regular rings. a ring R is said to be strongly \pi regular if for each x in R there exist a y in R such that x^n = (x ^(n+1))y. do...

31 December 2018 9,118 3 View

Large eddy simulation in film cooling?

I am recently working on a large eddy simulation in film cooling process. I would like to ask you to guidance me in this field. Due to the high number of mesh and small time steps in large eddy...

25 November 2018 9,318 4 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View