Dear Wiktoria!

Please You look at following articles:

Article Autologous Co-culture of Primary Human Alveolar Macrophages ...

Modelling lung inflammation treatment in vitro (attaached)

Article An in vitro triple cell co-culture model with primary cells ...

2.4. Triple cell co-cultures The co-cultures were prepared as previously described [5,12]. Briefly, epithelial cells (A549, 16HBE14o– and hAEpC) were maintained on transparent BD Falcon cell culture inserts (surface area of 4.2 cm2 , pores of 3.0 lm diameter, PET membranes for 6-well plates; BD Biosciences), in case of 16HBE14o– monocultures, the flasks and membranes were treated with fibronectin coating solution containing bovine serum albumin (for details, see description for monocultures). A549 and 16HBE14o– cells were cultured for 7–8 days, and hAEpC were cultured for 14 days, before MDM were added on top of the epithelial monolayer and MDDC underneath the insert membrane. Before using the triple cell co-cultures, they were kept overnight in medium supplemented with 1% L-Glutamine, 1% penicillin/streptomycin and 5 % heat-inactivated (pooled) human serum at 37 C in 5% CO2 humidified atmosphere.

For alveolar macrophage cell line, MH-S cells would be one option..

Article MH‐S, a Murine Alveolar Macrophage Cell Line: Morphological,...

Thank you for your answers! I went with primary cells co-culture. I had considered MH-S, but it wasn’t suitable for I was intending to do.