AcGFP1 vector can emit light under fluorescence without any reaction by substrate, nevertheless, Luc. vector is used which needs a substrate to be lighted.What are the advantages of Luc enzyme over GFP? For HL-60 transfection, since it is floating cell, which method would be the most effective and easiest?
One advantage of Luc over GFP is the sensitivity of detection. A tiny amount of expressed Luc is good enough to produce a signal above the background noise. Whereas GFP has to be expressed at relatively high level to make a clear distinction from an autofluorescence of cells.
To transfect HL-60 cells you could use either Effectine or Xfect.
Hope this helps!
All the best,
Manjunatha
One advantage of Luc over GFP is the sensitivity of detection. A tiny amount of expressed Luc is good enough to produce a signal above the background noise. Whereas GFP has to be expressed at relatively high level to make a clear distinction from an autofluorescence of cells.
To transfect HL-60 cells you could use either Effectine or Xfect.
Hope this helps!
All the best,
Manjunatha
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