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Questions related from S M Dew
I would like to differentiate THP-1 cell to macrophage using PMA and transfect pAcGFP vector with Lipofectamine ltx transfection reagent (as it is available). Is there any standard protocol of...
06 June 2017 6,049 3 View
I have PMA of 1mMolar concentration. I have to add 200nMolar of PMA in 0.5 ml cell suspension. So, what is the volume of PMA (in micro ltr) to be added in 0.5 ml suspension (as 200 nMolar...
06 June 2017 8,967 3 View
I want to perform the dual luc. assay using Hl-60 cell line which is a floating cell. Therefore, I explored different papers but no brief protocol found about the assay using this floating...
03 March 2017 9,698 1 View
I have got a sequence of my human promoter of interest-reporter (pGL4) vector of 3Kbp. The promoter sequence is in capital letters (representing the nucleotides) and the reporter vector sequence...
03 March 2017 3,177 5 View
I was trying to transfect HL-60 cells with plasmid containing AcGFP using Lipofectin in Opti-mem. But every time I failed. The cells were stained with DAPI. When observing under fluorescence...
02 February 2017 1,713 3 View
I want to explore the transcription factor candidate for a human promoter gene. I want to mean that, let I do not know about any possible factor. So, now how can I find a transcriptional factor...
02 February 2017 7,191 3 View
I need to transfect GFP vector and Luciferase vector plasmid DNA into the HL-60 floating cells. I will have to use X-tremeGENE HP DNA Transfection reagent from ROCHE. If anyone already experienced...
11 November 2016 5,007 2 View
AcGFP1 vector can emit light under fluorescence without any reaction by substrate, nevertheless, Luc. vector is used which needs a substrate to be lighted.What are the advantages of Luc enzyme...
11 November 2016 4,710 2 View
