Francesco Gubinelli

3 Questions 8 Answers 0 Followers

Questions related from Francesco Gubinelli

APOe isoforms Sanger sequencing - good primers and protocol?

Hello everybody. For my project i need to identify the APOe isoforms present on my patient derived lines. My idea was to extract DNA, amplify with PCR and send it for Sanger sequencing -...

25 April 2024 6,304 4 View

LPS genetic analysis variance between samples?

Hello everybody, i am trying to characterise my LPS model (rat) with qPCR analysis to check the level of neuroinflammation, but i have a pretty big variance between the LPS treated...

13 January 2018 4,807 2 View

DPSC and plate coating: what is better for differentiation into neurons-like?

Hello everybody. I'm trying to culture and differentiate murine DPSC into neurons like cells, but the proliferation ratio is really low. Moreover, present cells show different morphologies (no...

11 July 2016 2,313 4 View