We purchase aav1-syn-RGECO and RCAMP from addgene from the janelia's paper (Dana et al, 2006). We tried to transfect mouse primary, rat primary neuron culture as well as differentiated NSCs. We had low success in transfection and at times cell death. We had soem successful imaging, but most of the time the intensity was low, quickly bleaching even at 5Hz (50% duty cycle) and low activity in general (which can be improved with glutamate infusion).