Charles-Francois Vincent Latchoumane

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Questions related from Charles-Francois Vincent Latchoumane

Low transfection/cell death using synRCAMP/RGECO virus at different concentration and low imaging intensities: any tricks to improve the outcomes?

We purchase aav1-syn-RGECO and RCAMP from addgene from the janelia's paper (Dana et al, 2006). We tried to transfect mouse primary, rat primary neuron culture as well as differentiated NSCs. We...

30 April 2019 7,544 0 View

Primary neuron culture with and without astrocyte ?

Hi. I am diving in the new work of primary cell culture. I am culturing primary neurons from dissociated cells (Rat E18). My main purpose is to grow them, have them form a network and measure...

28 November 2018 8,918 1 View