Have anyone worked with cloning of short sequences for localizing the fluorophores to cell organelles such as vacuole, mitochondria, plasma membrane or any others?
I have done the same procedure with BL21 and pLysE but i got colonies but multiple times on rosetta i did but not getting. can anyone explain what would have gone wrong?
12 July 2024 9,904 1 View
I have a biomass with given ultimate and proximate analysis data. I am using Aspen plus for the simulation of gasification of biomass. In experimental data the gasifying agent air flow rate is...
18 June 2024 2,743 1 View
Space exploration presents unique challenges for human health, particularly concerning oral hygiene and overall well-being. The microgravity environment of space significantly impacts various...
24 March 2024 5,423 1 View
i am doing a project on gasification. during pyrolysis i got the following error: * WARNING COMPONENT 'BIOMASS' IN SUBSTREAM 'NC' HAS ZERO FLOWRATE IN THE OUTLET STREAM. FRACTIONAL...
18 March 2024 6,025 1 View
I am looking for the paper or the persons who worked on cloning of short sequences targeting the different cellular organelles (vacuole, mitochondria, plasma membrane, septum) in plant pathogenic...
11 March 2024 1,878 0 View
Dear all, I am doing a gasification of biomass. In the pyrolysis section i have used RSTOIC for the devolatization of the biomass. i have specified the yield from the RSTOIC. it gave me the...
19 February 2024 8,026 0 View
What is the difference between Mixed Methods and Triangulation Methods?
12 February 2024 9,962 3 View
I am working in validation and qualification of new HPLC method. Your insights and feedback are greatly appreciated.
02 December 2023 8,514 4 View
While testing the mediation via Process Macro in SPSS, I obtained that my Total Effect is significant, Direct Effect is also significant, but My Indirect effect is not significant. In this case,...
23 September 2023 6,641 1 View
CE=8.I.dt/(F.V.delCOD) Why Coulombic efficiency and COD removal is inversely related. If COD removal is high, the Coulombic efficiency is low and vice versa. Please explain the principle behind this
25 July 2023 5,717 2 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,526 4 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View
I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...
30 July 2024 3,294 3 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...
24 July 2024 5,789 0 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
When preparing a graph of fluorophore intensity vs. analyte concentration, the linearity shows an R² of 0.999. However, when plotting the same data as Fo/F vs. analyte concentration, the linearity...
13 July 2024 7,131 0 View
Hello, I am an Etch Engineer. I am performing etching with high T_ox/Nit selectivity using remote plasma. Etching plasma is formed using only NH3/NF3/H2. 1. I wonder if it would be advantageous...
11 July 2024 8,115 2 View