Dear eminent researchers iam planning to start IHC study in ameloblastoma using FAP ALPHA. Need your opinion?

Fibroblast activation protein (FAP )alpha

28 December 2020 8,101 0 View

What is the significance of hysteretic energy in RC member?

How can we use the energy demand of beams and columns or other structural memebers in real fileld? What are the applications of energy based design method?

25 November 2020 7,433 1 View

Which is the best machine learning method for topic extraction from large document collections?

I want to know about different algorithm/method/inference to extract underlying topics/themes from documents/scientific article etc.

12 October 2020 5,911 3 View

The porous nature of the material should show up in the impedance behaviour in terms of inductance element?

The porous nature of the material should show up in the impedance behaviour in terms of inductance element. I do not see any value given for the inductance at the interface in these plots which...

01 October 2020 7,504 2 View

Could someone suggest good journal/article realted to energy dissipation through hysteretic behaviour?

Currently I am working on " Quantitative Assessment of dissipated energy from structural components correlation with deformation under seismic loads in low rise building using Perform 3D".

13 July 2020 3,330 2 View

Does anyone know whether NCI-H522 and NCI-H1437 lung cancer cell lines grow as xenograft in nude mice?

Please let me know whether any body in this forum has first-hand experience growing them in nude mice. I was thinking about injecting 5 million cells in 1: 1 ratio of Matrigel and PBS. Let me know.

07 February 2020 4,661 1 View

Is there any way to separate MoS2 QDs from MoS2 flakes?

I have tried to exfoliate MoS2 via liquid exfolaition method(using NMP as well as Ethanol/water mixture as solvent) . Always Iam getting MoS2 nanoflakes and MoS2 QDs. Is there any way to separate...

03 February 2020 6,771 1 View

What would be a suitable econometric method to evaluate socio-economic impact of social security ?

Research methodology

25 January 2020 7,278 2 View

I want to coat Ionic liquid over Fe3o4@Sio2. I have very concentrated only 0.19g of ionic liquid. Does anyone have idea how can i do it?

I have successfully coated the Fe304@Sio2@PPy( Polypyrrole) simply by taking 100mg Fe304@Sio2 ,suspended in 15 ml DI water then sonicate for 15 min then add 100 microlitre pyrrole. Solution was...

13 January 2020 8,206 5 View

Wether it is possible to dissolve Amyloid beta 1-40 monomer with phosphate buffer or do we need to HFIP?

I have Amyloid beta 1-40 monomer using which i need to perform biophysical characterisations.

16 September 2019 4,472 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Why does two different bioinformatics tools for the same proteins show different subcellular localizations?

During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...

01 March 2021 6,729 2 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

No title

17 February 2021 4,217 3 View