Hi,
I am working with a lncRNA (uncharacterized ) which i found to be present within the intonic region of my gene in human. When i blast it with mouse genome i did not find any sequence corresponding to it, assuming that it is absent in mouse.
However, by mistake i used 2 mouse cell line cDNA and used the primers for my lncRNA, i got exact size band. I thought of sequencing it and to my surprise it came out to be my LncRNA.
My question is
1. Is it possible that i identified a new lncRNA from mouse and i am unable to detect it in mouse genome as only 95% of mouse genome is sequenced and lncRNA falls in non coding region?
2. What can be the other possibility for this weird observation.
Thanks
Atoshi