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Questions related from Atoshi Banerjee
Hi, We purchased tm1b mice where homozygous mutant is lethal so we have Het and corresponding WT. When i am trying to geneotype i see a higher band indicating presence of distal LoxP site in all...
08 July 2019 3,544 4 View
Hi, I am working with a lncRNA (uncharacterized ) which i found to be present within the intonic region of my gene in human. When i blast it with mouse genome i did not find any sequence...
20 April 2019 2,188 3 View
Hi I am trying to express my lncRNA in neural cells. As transfection efficiency will be low i am planing to use lentivirus as my delivery method. But to be sure can i add a GFP protein using IRES...
25 March 2019 8,465 8 View
Hi All, I am trying to access changes in B cell markers in our knockout mouse and wild type from blood. I try to gate lymphocytes, then single cell, followed by live dead and B220+ cells. When i...
28 February 2019 1,283 2 View
Hi I want to do NGS for microRNA presents in my exosomal samples obtained from cell culture. I have isolated exosomes followed by total RNA extraction. However, when I checked the quality on the...
09 December 2015 3,947 3 View
