7 Questions 2 Answers 0 Followers
Questions related from Aj Keefe
Hi I'm using LysoBrite (a variant of Lysotracker) for live cell imaging with iPSC derived neurons. The problem I am having is that it bleaches fairly rapidly (within ~1 minute of imaging) and the...
09 June 2021 2,744 0 View
I understand Mycoplasma can fly under the radar, but are other forms of contamination obvious over extended culture? I have been growing neurons for 30 days, and since there are no obvious...
06 March 2021 4,273 4 View
Hi, I'm doing some live cell imaging experiments with SHSY5Y cells. Could someone pass me their media formulation?
23 September 2020 9,094 3 View
Hi, we are generating primary neurons - I was curious if the remainder of the brain could be fixed in PFA and used for IHC analysis without prior perfusion fixation?
22 September 2020 4,423 3 View
Hi, I've read numerous studies that treat SHSY5Y cells with 100-1000 uM FeCl2. In these studies, there is no mention of how this solution was prepared. I assumed they simply added iron chloride to...
10 August 2020 2,949 1 View
I understand bacteria growing in media, but how do bacteria survive and grow on the outside of culture flasks? What is their source of nutrients? And if there is growth on the outside of a...
23 November 2019 4,363 0 View
Since we have detailed data of some strains of mice, such as C57BL/6, from experiments dating to almost 100 years ago, I wondered - do mice we use in experiments today that are derived from this...
01 January 1970 3,619 1 View
