Lysotracker bleaching and toxicity (with movie!)?

09 June 2021 0 3K Report

I'm using LysoBrite (a variant of Lysotracker) for live cell imaging with iPSC derived neurons. The problem I am having is that it bleaches fairly rapidly (within ~1 minute of imaging) and the lysosomes seem to abruptly stop moving after ~ 10 frames (10 seconds).

Below is a link to a movie. You can see movement (and brightness) starts high, but everything slows downs and dims over time.

https://youtu.be/ZtAWkPtMk8I

Any advice is appreciated!

Thank you!

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