Adam M. Farkas

5 Questions 11 Answers 0 Followers

Questions related from Adam M. Farkas

Does anyone have a good protocol for staining bacterial cells with a primary Ab followed by a fluorescently-conjugated secondary Ab?

Hi All, Does anyone have a good protocol for staining bacterial cells (in suspension culture) with a primary Ab followed by a fluorescently-conjugated secondary Ab? I'm just wondering primarily...

03 March 2015 801 2 View

How many CXCR5 single positive CD4 T cells are normally present in Spleen/LNs of non-immune mice?

Hi all, Does anyone know approximately what percentage of CD4 T cells in the Spleen/LNs of non-immune mice are CXCR5 single positive? I know in the Peyer's Patches a high percentage of CD4s are...

09 September 2014 9,074 1 View

Does anyone have knowledge on genotyping for APCmin mice?

Hi All, Does anyone have a good protocol for genotyping APCmin mice? I have the primers from the paper below, but was wondering if anyone has recommendations for the PCR setup (i.e. annealing...

07 July 2014 2,753 7 View

What is the best orientation to cut sections of mouse intestine?

Hi All, Does anyone have a preferred orientation in which to section mouse small intestine so that one maximizes the number of intact villi in the section? I have tried paraffin-embedding the...

07 July 2014 4,181 4 View

Has anyone had experience staining 5-6um paraffin sections with toluidine blue?

I know toluidine blue is used to prepare plastic-embedded thin sections for EM. Has anyone used as a bacterial nuclear stain on thicker (5uM), formalin-fixed, paraffin embedded sections?

06 June 2014 794 5 View