i need the protocol for lignin composition y HPLC method, complete procedure with all the reagents used and standard used. thank you?
Lignans are a complex mixture, you'll probably need different colums to purify them.
One source:
NMR Applications for Botanical Mixtures: The Use of HSQC Data to Determine Lignan Content in Sambucus williamsii | Journal of Natural Products (acs.org)
Hi
Chemically lignin is polymer so maybe Waters application will be useful as a basic source:
https://www.waters.com/webassets/cms/events/docs/GPC%20Event/2016/Related%20Techniques/2016%20GPC_Taihyun%20Chang.pdf
or papers like this
Lignin Analysis by HPLC and FTIR
https://link.springer.com/protocol/10.1007%2F978-1-4939-6722-3_14
Best regards
Tomasz
Dear Pirzada,
in case you are interested in the aromatic compounds in depolymerized lignin, I can recommend our HPLC method described here:
Article Enabling the valorization of guaiacol-based lignin: Integrat...
The method uses a C18 column and acetonitril as mobile phase coupled to a DAD detector and can separate protocatechuate, benzoate, 4-hydroxybenzoate, phenol, catechol, p-coumarate, syringate, guaiacol, alkylated catechols, vanillate, ferulate, vanillyl alcohol, caffeate, cresols (m, o, p), and many more.
Best
M
https://doi.org/10.3390/plants6040042
https://doi.org/10.1007/978-1-4939-3185-9_26
https://doi.org/10.1016/j.ultsonch.2011.06.018
This source i hope it'll help you Dr
https://pubs.acs.org/doi/pdf/10.1021/jf00088a036
As per the widely used NREL procedure for determining biomass composition (you can just google it!), HPLC comes into the picture only when determining the sugar composition of the biomass. Whereas, lignin content is already determined in earlier steps. This is because, lignin, even when depolymerized, will not completely dissolve in water and hence cannot be eluted through HPLC- it will most likely damage the column.
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