What is the best program to simulate temperature of the tool tip during turning (Abaqus or Ansys)?

Dear all, I would like to simulate the temperature of a tooltip during turning. What program should I use best? And does anybody know where I can find a template where I can start from? This...

28 June 2020 2,572 3 View

How to do size reduction of DICOM CT pixeldata?

Hi all, I'm hoping you could help me with a conundrum regarding reduction of size of dicom CT pixeldata. I'm using the Cornerstone DICOM parser (https://github.com/cornerstonejs/dicomParser) to...

02 March 2019 651 6 View

How do you remove a oxidation layer after laser hardening?

Hi! I am preforming laser hardening test on Stavax Steel with a certain texture. On this texture forms a black oxidation layer after the laser hardening treatment. How can i remove this...

25 February 2019 7,503 1 View

What are the most important insights and texts in contemporary studies of rhetorical ethos, credibility, and trust?

19 February 2019 601 7 View

Does anybody know a good online editorial tool in english for academic writing?

I have tried prowriting.com but I am not convinced. Any hint would help. Thanks. Jens

04 January 2019 3,375 5 View

Does political correctness have an impact on necessary controversial debates?

I am experiencing a lot of "self censorship" particularly in US companies these days. Problem is, that even necessary debates about changes and (revolutionary) ideas quite often seem to drown in...

20 September 2018 4,610 4 View

How to I add a ruler to various images dependant on position?

I'm automatically capturing images of a specific profile, which varies a few millimeter in position and angle. I am looking for a solution which can automatically detect the left and right edge of...

30 May 2018 7,245 3 View

Does anybody know someone who does research in connection to archetypes and branding?

Related topics are brand personalities, social psychology, cultural anthropology, maybe consumer culture. Thanks Jens

29 March 2018 3,145 10 View

Which international journals would be appropriate for publishing research findings concerning archetypes and branding?

Maybe journals which publish marketing related topics in regards to social psychology, cultural anthropology or consumer culture theory?

20 March 2018 6,420 3 View

How to pull very sharp microinjection needlles?

30 May 2017 2,377 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

Best solvent for L-DOPA extraction and HPLC analysis?

I'm working on Mucuna pruriens and want to quantify L-DOPA. Could someone please suggest the cheap and best solvent for the extraction and the solvent system that has to be used for its...

20 February 2021 6,058 2 View

QPCR tubes for Roche Lightcycler Nano?

We own a small cPCR thermal cycler from Roche, Lightcycler Nano, which is convenient for backup quick runs. Recently, Roche has discontinued the plastics for it and they offer no alternative from...

17 February 2021 8,694 5 View

I have been reading a lot about PCR and still I am ending confused. I need to know the difference between RT-PCR, real-time PCR, qPCR, RT-qPCR ?

I Know that RT-PCR is Reverse Transcription PCR when the starting material is RNA. Going for reverse transcription first (RNA to cDNA) and then it could be conventional PCR with the need for gel...

11 February 2021 6,852 4 View

XRPD Quantification - Building a calibration curve of drug excipient?

I am aiming to utilise XRPD to quantify a drug excipient within a drug product and have been having a little trouble building the calibration curve to allow for this quantification up until this...

10 February 2021 6,145 3 View

What is the general miRNA yield one gets from the Qiagen miRNEASY serum/plasma advanced kit?

I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...

02 February 2021 8,806 2 View

Can I heat my ELISA at 37C?

Hello, I am conducting a sandwich-type ELISA. It is to see if small molecules can interfere with a protein-protein interaction. As this interaction would occur in the human body, may I heat...

31 January 2021 4,271 3 View

I have some "stupid" questions about the Western Blot, can you help me?

I started learning the Western Blot a few days ago and I have an okay understanding of the process, but there are a few steps I don't understand how to do by myself from scratch. I want to...

25 January 2021 6,691 8 View