Receiving very less number of hits and events in acoustic emission tests?

I am doing a three point bend test with concrete specimen of dimension 280mm*90mm*90mm. I am putting 2 sensors near the notch with 3cms away from both the sides. the experiment ran for 20 mins but...

13 February 2021 2,990 1 View

How do I use the NIST test suite to test the randomness of an RGB color image?

I would like to use NIST test suite for Cryptographic Applications on a color image to test whether the image is random or not. But I don't know how to do all these tests. I used some...

06 April 2020 2,021 1 View

Are there any difference between : R and DEM (pysical model )?

R and DEM (pysical model )?

13 February 2019 10,010 3 View

Can you kindly please give me what are the best topics to answer this question: (oil price and the affect on the economy)?

Which countrys should i talk about and what different topics

07 August 2018 1,799 3 View

How can I start writing a Matlab code for making LDPC code, and how to compare with Turbo or any other codes?

I am doing PhD, and I need to create an LDPC codes for my initial understanding

03 February 2016 2,490 3 View

No title

I am wondering if anyone has used the pre-optimized primer pairs from Genecopoeia for RT-qPCR. I am considering these for some mouse transcripts.

02 February 2015 2,795 2 View

Could someone help with an in-vitro osteoclastogenesis assay protocol (from whole bone marrow culture)?

I'm planning to set-up an in-vitro osteoclastogenesis assay from mouse bone marrow culture, followed by TRAP staining. Could someone share a detailed, working protocol for that? Any suggestions...

06 July 2014 7,687 15 View

How to manage Odontology University classes in times of covid 19

Many countries had their Universities classes interrupted due to covid 19 pandemia. Now we have to prepare ourselves to come back to our classes at University. Theoretical classes seem easier to...

01 January 1970 5,410 1 View

No title

03 March 2021 5,360 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View