Samra Sardar

6 Questions 23 Answers 0 Followers

Questions related from Samra Sardar

Lentivirus based shRNA or CRISPR/Cas9 technology for knockdown/out a transcription factor in primary mouse B lymphocytes?

I want to study the effect of a particular transcription factor on activation and proliferation of B lymphocytes. My aim is to knock-down or knock out the transcript in primary B lymphocytes...

22 February 2017 2,183 2 View

Is it possible to extract total RNA from whole tissue lysate prepared in RIPA buffer and stored at -80 degree?

I have mouse joint tissue lysate prepared in RIPA lysis buffer + protease inhibitors + phosphatase inhibitors, for western blotting. Now I want to run qPCR in parallel to WB but don't have any...

25 February 2015 5,597 2 View

Anyone having experience with Genecopoeia All-in-One™ qPCR Primers for mouse transcripts?

I am wondering if anyone has used the pre-optimized primer pairs from Genecopoeia for RT-qPCR. I am considering these for some mouse transcripts.

03 February 2015 2,930 2 View

Are Cathepsin K-Cre (OC specific) and Osterix-Cre (OB specific) mice available?

I am interested to know if osteoclast specific Cre mouse (Ctsk-Cre) and osteoblast specific Cre mouse (Osx-Cre) are available from Jacksons or any other source and also the price for each. I have...

17 December 2014 9,210 3 View

Is it possible to have a candidate gene with no coding SNPs but differential levels of methylation?

I am working on a genetic region (quantitative trait locus) that was identified in linkage analysis between two mouse strains that differed in response to induced arthritis. I am trying to...

18 October 2014 7,718 8 View

Could someone help with an in-vitro osteoclastogenesis assay protocol (from whole bone marrow culture)?

I'm planning to set-up an in-vitro osteoclastogenesis assay from mouse bone marrow culture, followed by TRAP staining. Could someone share a detailed, working protocol for that? Any suggestions...

07 July 2014 7,831 15 View