Dear Emmanouil Charakopoulos

I have a suggestion.

You can measure interleukin concentration in serum or plasma by yourself as it does not require much expertise by using ELISA kits for measuring the interleukin of interest. You just need to purchase the commercial kit, and follow the protocol as stated in the kit insert. It does not require experience.

Additionally, you will need a set of pipettes like P10, P20, P100, P200 and P1000, multi-channel pipette (for washing), ELISA washer (optional), ELISA shaker and ELISA reader, and 4 degree refrigerator to store the kit. All the reagents and the 96-well coated microtiter plate or strips will be provided in the kit.

Just run a set of standards mentioned in the kit insert by serially diluting the standard provided in the kit to obtain the standard curve. Use your samples as is or dilute your samples 1:10, etc., with the assay diluent provided in the kit so that the absorbance of your samples come well within the standard range. Calculate the interleukin concentration from the standard curve. If you have diluted the samples, you may multiply the concentration of the samples obtained from the standard curve with the dilution factor to give you the final concentration of the original undiluted samples.

I am sure you may be having micropipettes and microtips. As far as the instrumentation is concerned you may always request another lab to allow you to use these instruments for a day as ELISA is a one/two-day procedure.

I would suggest that you do the procedure yourself as it would save time as well as money.

Good Luck!

Dear follow the below paper https://www.sciencedirect.com/science/article/abs/pii/002432058890522X