Hello dear scientist,
I am looking for an easy (kit?) way to extract total protein from soybean seeds.
There are procedures and reviews (Preece et al., 2017) available, but mostly for large scale, like tofu production etc.
I was wondering if anyone has good experience with e.g. the P-PER Plant Protein Extraction Kit:
• Versatile—works with multiple plant organs (leaf, stem, root, seed and flowers); multiple plant species (Arabidopsis, tobacco, maize, soybeans, peas, spinach and other plant tissues); and fresh, frozen and dehydrated plant tissues
Since no mechanical disruption is part of the protocol I don't think it would be too suitable for seeds...? Can anyone recommend a protocol which worked in your lab?
Thank you in advance,
Elisa
Please find enclsoed some relevant links....
QuickStix Strip Test - Roundup Ready Soybean Seed www.biotech.iastate.edu/.../QuickStix-rr_soybean* 2 different varieties of soybean seeds, ... The QuickStix kit detects the CP4 EPSPS proteinproduced in the seed. To conduct the test, a seed sample is placed in a ...
Grain Protein Testers and Meters | Graintec Scientific
www.graintec.com.au/products/grain-testing/protein
Graintec Scientific is a supplier of testing equipment to the food and agricultural industries. Near Infrared Analysers NIR, Protein testers on-farm,Water Activity .
Dear Elisa
There are no kits available to extract the protein rather kits will be used for the analysis of protein. There are lot of extraction method for protein and the following links would be helpful for you.
https://www.sciencedirect.com/science/article/pii/S1466856416305823
https://naldc.nal.usda.gov/download/IND43969201/PDF
http://share.iit.edu/bitstream/handle/10560/762/extractionofprot00lazo.pdf?sequence=1
http://www.fao.org/docrep/t0532e/t0532e07.htm
http://onlinelibrary.wiley.com/doi/10.1111/j.1745-4549.2009.00400.x/abstract
https://patents.google.com/patent/US4704289
The below mentioned article, on soy bean protein, may provide some answers. Best wishes.
https://www.researchgate.net/publication/265971321_Response_of_pulps_of_different_origins_to_the_upgrading_effect_of_bulk_added_green_denatured_soy_protein_in_correlation_to_morphological_structure_chemical_composition_of_cellulose_fibers?_sg=b-tlhCL5ExiAt1nOotVM80LvtmpqEkB-ySnnqykGLUrxYiF4v-2VLN41q-Zy1S4a-owNQu-rVczO0_J8mzd24LS8_CgBKZeWQfih_xW7.uCf-_-y9MavcOdKoYMRPZ3oHe-5-p5pl0E-IqWRbOquIB6uUb0D_ix4uuYAyjkWL-ffClpubnyP8EWLOYOiqrw
I need to extract proteins from wheat seeds for gel electrophoresis. anyone can suggest me which is best process.
- Badges
- Science topic
- Similar topics
- Biological Science
- Biota
- Eukaryota
- Fungi