Could anyone share a Ki67 staining protocol for MCF7 cells, I have tried one but it didn't yield the right results?
And also the cell conditions, because I read that cells has to be in a certain phase when used for this technique.
When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!
29 July 2024 488 0 View
In most colorimetric determinations of methylglyoxal, there is neutralization using saturated potassium carbonate, and perchloric acid is added to the final reaction. I would like to understand...
23 June 2024 2,074 0 View
How to stabilise the Schiff base product and avoid it from reverse back to the aldehyde and primary amine.
09 June 2024 9,264 5 View
For different initiators having different half life at different temperature, which half life is more practical for polymerization temperature control ? such as ten minutes, or 30 minutes half...
08 June 2024 3,104 6 View
I don't know how to use the Hall-Patch equation for Titanium alloys that contain two phases.
29 May 2024 3,664 1 View
We use DpnI to digest PCR product(already purified),then after digesting,do I need purified again?
09 May 2024 2,499 3 View
Hi, I'm trying to find a pDNA transient transfection carrier for my MDA-MB-231. I'm using either liposome or lipopolyplex, but the transfection variability between experiments are too great. I can...
28 April 2024 1,189 2 View
If so, do the input ligands need to be protonated with SMILES strings?
09 April 2024 5,547 1 View
I performed multiple passes of stir friction machining of AZ31 magnesium alloy (average grain size 12μm) under different cooling media. The results show that the average grain size can only be...
07 April 2024 6,848 0 View
This question is well answered now. Thanks!
07 April 2024 6,030 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...
25 July 2024 7,520 0 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...
19 July 2024 3,906 0 View