Transduction of mouse primary neurons with AAVs, protocol?

Hello there, I am culturing cortical neurons from E17 mice and will need to transduce them on DIV7. Does anyone have a protocol on how to do so best? Any ideas on MOI? Im using CRISPRa plasmids,...

10 December 2020 7,900 2 View

How do I blot an 8kDa protein?

Hi, I am trying to blot a protein of 8kDa with western blotting. So far I have tried to use 0.45um & 0.2um nitrocellulose membranes for transfer as well as 0.2um PVDF. I have tried to use...

01 October 2019 7,741 5 View

Enrichment of spermatogonial stem cells from C57BL/6 mice?

Dear reader, I am searching for a protocol on isolation and enrichment of spermatogonial stem cells (SSCs) that is suitable for C57BL/6 mice without a mixed background. As far as I have been...

04 September 2018 2,559 3 View

Changing the elctrolyte solution on pH-electrodes with lab made solutions?

Hey there, our electrolyde solution in the pH-electrode is low (its a double junction electrode). We looked up the price for new solutions and the commercial ones are not super cheap and in the...

23 November 2016 6,727 2 View

Can I eliminate items from a long scale to conduct a survey ?

I am combining different scales in my questionnaire. These scales have been tested for their validation and their reliability. 1st. Do I still need to examine discriminant validity? 2nd. The...

30 October 2016 7,545 4 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

What role should journals play in leading academic fields?

A recent paper by Yeung and Thomas (2021) on journals in event management opens up an interesting debate on the role of journals as academic fora. They demonstrate that event management publishing...

28 February 2021 3,494 6 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Where i will get the Desmodium and Napier grass seeds?

26 February 2021 975 4 View