Why does sodium metabisulfite generate turbidity when mixing with sodium hydroxide?

When I tried to mix a sodium metabisulfite solution with sodium hydroxide solution, the mixture solution quickly became turbid. Before mixing both were clear solutions. Please help explain the...

03 February 2021 8,075 2 View

Any suggestion for conductive paste for PDMS or Ecoflex for electrode?

Dear colleagues, I am trying to make a sturdy electrode on a flexible polymer substrate (PDMS or Ecoflex). I have used a silver paste, but once cured, it peels off very easily. the cured silver...

14 December 2020 4,236 3 View

Transduction of mouse primary neurons with AAVs, protocol?

Hello there, I am culturing cortical neurons from E17 mice and will need to transduce them on DIV7. Does anyone have a protocol on how to do so best? Any ideas on MOI? Im using CRISPRa plasmids,...

10 December 2020 7,900 2 View

Are there any easy way to test the activity of CYP51?

I'm looking for a relative easy way to test the activity of CYP51. Are there any way that I can measure it without using LC-MS or gas chromatography. A simple absorbance or fluorescence test will...

08 October 2020 6,687 3 View

Can we use HR-ICP-MS to measure As?

Hi there, we have the ICPMS element 2 using argon gas. I am wondering whether we can use element 2 to measure arsenic? Can you please provide more information or papers that I can check? Thanks!

16 August 2020 1,142 3 View

Where to publish systematic review protocol?

I would like to prepare a systematic review protocol. I am wondering about any journals that will publish a protocol without charges from the authors? It does not have to be open access. The topic...

23 July 2020 4,537 8 View

How to perform western blot for Toll-like receptor 3?

I encountered some problem to detect TLR3 for my western. I did not get any bands. After reading a bunch of literature, I am not sure exactly what I did was wrong. I appreciate if anyone has...

19 July 2020 9,402 3 View

How to minimize non-specific binding of eGFP protein to magnetic nanoparticles?

I am working on protein conjugation into nanoparticles. Magnetic nanoparticles were washed with DI water and TBS buffer and then combined with eGFP fused protein for 24 hrs. After removing the...

01 July 2020 8,864 3 View

Where to find recovery rate and death rate of COVID-19?

Some people, even doctors say it's not a serious disease, it's like seasonal flu, which could cause death. Where to find the recovery rate and death rate of COVID-19 in respect of older adults...

16 June 2020 6,546 12 View

Can we only capture one diffraction order in light modulator?

Obviously, the spatial light modulator (SLM) and digital micromirror device (DMD) is diffration gratings. Therefore there would be multiple diffraction orders of the created images. Here I want...

06 May 2020 396 8 View

How do you write a simplified C block code of this average-current control on PSIM?

This is a Buck (step-down) Converter and I am new to this program. I need to program with simplified C block and I actually don't know what to do. Can anyone show me how to do this? Or at least...

02 March 2021 8,233 1 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Can I download BibExcel for MacOs?

Hi everyone, I would like to ask how I can download BibExcel software for MacOs? There are some links which are supported by Windows but I could not find it for Mac. or is there any other software...

01 March 2021 652 1 View

Is it possible to set a breakpoint at a specific DYNAMIC instruction using the GNU Debugger (GDB)?

Using GDB, it is straightforward to debug and monitor a target program by setting a break-point at a specific instruction, since the instruction addresses are known. However, we have two...

24 February 2021 1,598 2 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

Anyone with some Transgenomic Wavemaker software? Seeking help from the chromatography community?

I am trying to seek out some Transgenomic Wavemaker software (happy to purchase a computer too) to operate a fraction collector. The only way to connect to the fraction collector so far is to...

23 February 2021 9,181 1 View

Is it the right method to transform path coefficients into correlations before meta-analysis?

We all know it is possible to get path coefficients from correlation matrix (e.g., PATH ANALYSIS I: INTRODUCTION (ecu.edu) ). Using a simple solver on a spreadsheet, it is therefore possible to...

23 February 2021 8,366 3 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

Can I use FLEXBAR to detect and remove barcodes out of my short reads without demultiplexing ?

Hello, I have demultiplexed my short reads and I have removed the Illumina adapter left in the sequences and now I need to remove the barcodes used by the sequencing centre from my reads. I have...

22 February 2021 9,873 3 View