I need this to quantify the peptide by HPLC methods (analytical assay).
The peptide is 900 Dalton.
The Dextran is 70000 Dalton.
https://www.ncbi.nlm.nih.gov/m/pubmed/26773880/
This might help i think
Dear Hafiz,
when we have such separation problems, we use specific "centrifugal filtration devices". Their use is very easy. The have the size of an Eppendorf tube and fit in every normal centrifuge. Inside they have a filter cartridge with a filter membrane, which has a certain size exclusion. As example, Millipore has an Amicon4 with a 300 Dalton cut-off so only your peptide will pass through. Other suppliers have similar devices, where you can select your cut-off of choice. Simply check out for "centrifugal filtration devices".
Good luck,
Christoph
If possible you can try Size-exclusion chromatography (SEC), or molecular sieve chromatography
The content of peptide is more than 1% and hence extraction with solvents like ethyl acetate or dichloro methane, followed by crystallization with hexane should help for purification.
There are several reported methods for the assay.
The methods are selective for the class of peptides. You need to choose from that.
Dear Christoph Wittmann and Bojan Sarkanj ,
Thank you for you suggestion. I'll try it.
Dear Mohandoss Thammanna ,
Thank you very much.
Do you have reference to start with?
There are several reported literature on purification of peptides and aminoacid derivatives that captures this kind of extraction.
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