according to me LCB and PICRIC ACID staining followed by microscopy is the best methodology to follow mycelial and sporulating structure of fungi including penicilliunm for identification. If required you can follow the slide culture technique for intact structures.

Very thanks for answers.

Hero suggesting Czapka? To me the fungi grow poorly on this medium.

Ok I try Czapek with yeast extract.

best regards

better use PDA . If you are using CDA supplement the medium with soil extract for better growth of the Penicillium sp.

Yes, on PDA medium grow very good.

Penicillium give good colony characters on Malt extract agar, Czapek and Czapek yeast agar.

Pitt JI (1979) The genus Penicillium and its teleomorphic states Eupenicillium and

Talaromyces. Academic, London. I used this book for morphological characterization of Peniciilium species.

All mentioned agars are good for Penicillium morphology - recently,  YES and CREA were also used for Penicillium identification. I recommend taxonomic studies published in the Studies in Mycology (Penicillium subgenus Penicillium: new taxonomic schemes and mycotoxins and other extrolites and Phylogenetic and taxonomic studies on the genera Penicillium and Talaromyces) for Penicillium identification.

Very thanks.

For identification  of Penicillia

The original monograph by C Thom " The Penicillia" The Williams & Wilkins Co. Baltimore USA 1930. The description of Penicillia and identification based on morphological & microscopic characteristics.

The latest I saw is Identification & Nomenclature in the genus Penicillium. Studies in Mycology. Vol 78. 343-371. Published by C.M. Visagie et al. They have given current list of penicillia based on morphological, microscopic and DNA Sequencing studies.

Good Answers Dr Ghanshyam

i need (Studies in Mycology. Vol 78. 343-371. Published by C.M. Visagie et al. )