ITS1 region sequence

More Asma'a Olwey's questions See All

Morphometeric analysis using mixed data?

How can I get a data matrix using mixed quantitative and qualitative (binary and multistate) data scoring?

31 December 2017 978 7 View

How to study the phylogeny of different species in genus Heliotropium?

I tried to study the DNA sequences of different species of genus Heliotropium, I first used the following primers, ITS1 primers Baldwin (1992) P1 (5'-TTC AAC GAG GAA TTC CTA GT-3') and P2...

10 November 2013 2,834 6 View

What are the best primers used for Boraginaceae phylogeny?

Can anybody advise me with the best primers to be used for phylogeny of family boraginaceae especially genus Heliotropium. What about universal primers, will those give valuable information?

10 November 2013 9,349 3 View

How to be sure my extracted DNA is pure?

I'm trying to extract DNA from plant herbarium specimens, but I want to disinfect the plant leaves first so the extracted DNA is pure (not contaminated by fungal or bacterial DNA).

04 May 2013 1,215 5 View

Is there any way to calculate whole genome similarity of organism without the whole genome sequencing ?

is there any ways that i can find genome similarity of an organism without whole genome sequencing ,like using maths formulas or experimental progress ?

05 July 2024 4,070 3 View

Have pathomorphological studies of the lungs been performed in patients with desminopathy?

It is known that patients with desminopathy often die from pneumonia. Have pathomorphological studies of the lungs been performed in patients with desminopathy?

15 June 2024 1,355 7 View

How can I decide the variant I found segregating with a genetic condition is novel or known?

I found a variant segregating with a genetic condition, this variant is listed in ClinVar (with no clinical significance), gnomAD, etc. However, I could not find any publication reporting the...

10 June 2024 7,606 3 View

Describe the principles of Population Genetics and evolution as applied to livestock breeding programs?

Describe the principles of Population Genetics and evolution as applied to livestock breeding programs

08 June 2024 2,924 0 View

What is the suitable concentration of hygromycin in tomato sp. Micro-tom genetic transformation?

I used hygromycin and kanamycin for selecting positive buds in Micro-tom genetic transformation. Kanamycin performed very well and I could get positive plants soon, but hygromycin is a little bit...

29 May 2024 6,911 0 View

Are there any literature sources for proving that Geotrichum silvicola colony morphology can be slimy?

Hi, I've recently come across the colony of Geothrichum silvicola (based on sequencing result) isolated from soil with slimy morphology, unlike the colony morphology I've found on the journal...

13 May 2024 1,356 8 View

Will this vector plasmid works well?

Hello I am currently engaged in research focusing on plant genetic transformation. As part of this endeavor, I have designed a comprehensive in silico plasmid cloning approach. The aim is to...

07 May 2024 5,119 3 View

Why I am not getting accumulated GFP in vacuole for om45 WT GFP yeast strains?

I am following the standard method which is mentioned in Tomotake Kanki et al., 2009 research paper Monitoring mitophagy in yeast: The Om45-GFP processing assay . I am growing the cells in SD-HIS...

04 May 2024 6,463 0 View

Who (first) proposed/used/coined the term ‘translation’ in biology/genetics?

Who (first) proposed/used/coined the term ‘translation’ in biology/genetics? What is the history behind the use of the word? Thank you!

01 May 2024 4,528 10 View

Can you help in Linkage Analysis Tool?

Can anyone assist with running the Linkage Analysis Tool 'Easylinkage' or any alternative tool for conducting linkage analysis and calculating LOD scores?

06 April 2024 7,273 2 View

Dr Ghazanfar Ali

Dear Asma, you can get sequence of ITS1 from gene bank database for your further process. But please clear your need in good way. Then any person can help you. Thanks

Rolf Henrik Nilsson

https://www.researchgate.net/publication/252931467_Improved_software_detection_and_extraction_of_ITS1_and_ITS2_from_ribosomal_ITS_sequences_of_fungi_and_other_eukaryotes_for_analysis_of_environmental_sequencing_data?ev=prf_pub

Article Improved software detection and extraction of ITS1 and ITS2 ...