hi Asmaa

I think ITS - sequencing is the best. Please download and see this paper: http://www.amjbot.org/content/89/2/287.full.pdf+html

good luck

Hi Asma'a,

We have used both the nrITS and the trnK intron in Myosotis. Both provide suitable levels of resolution for our purposes. See this paper for details: https://www.researchgate.net/publication/11237191_The_origins_and_evolution_of_the_genus_Myosotis_L._(Boraginaceae)?ev=prf_pub

At the level you seem to want to work at the nrITS is likely to be useful but if you also want a chloroplast marker you may need to experiment with primers for several in order to find one or two that are suitable for your situation. This paper includes a number of possibilities:

http://www.amjbot.org/content/92/1/142.full.pdf+html?sid=a7b9c5d3-174b-410f-924b-91d3051b3a26

All the best

Article The origins and evolution of the genus Myosotis L. (Boraginaceae)

Hi Asmaa,

We worked on Cynoglosseae using ITS and also trnL-F. Those gave reasonable resolution at tribal level. I would suggest to you to perform a variability test prior starting with massive amplifications. We found some problems amplifying herbarium material, that we solved using internal primers.

Best