How do I assess when to use "match between runs" for LFQ analysis on Maxquant?

Hi Proteomics enthusiasts, I am currently analyzing DNA pulldown data (treated vs non-treated) using Label-free quantification on Maxquant. I am trying to assess if a protein is present in one...

04 August 2020 1,090 6 View

Why am I unable to detect Histone PTMs at their correct molecular weight in nuclear extracts via Western Blotting?

I am trying to detect changes in Histone PTMs with or without treatment in nuclear extracts from cell lysates. I use a Hypotonic buffer (20 mM Tris pH 7.4, 10mM NaCl, 1mM MgCl2, 0.5mM EDTA)...

10 March 2020 2,626 6 View

Conceptual/Theoretical model of participation in mutual fund / microcredit?

I am looking for a theoretical or conceptual model of participation in mutual fund or microcredit group. Although there are several empirical research about drivers and determinants linked to...

11 February 2020 5,179 3 View

Has anyone else seen a reversal of solutions during an mRNA extraction?

I am trying to do an mRNA extraction to be used in qPCR. I am using trizol. When I add the chloroform, I should see a clear aqueous solution containing my mRNA at the top and the pink phenol...

10 April 2019 7,821 3 View

Specific primers for marine chytrids/oomycetes?

03 September 2014 8,892 3 View

What is the best software for storm water flood inundation mapping?

I'm modeling a storm event hydrologic responses of urban watershed using fully distributed hydrological model? In this modeling I want to generate the spatially distributed runoff values in the...

20 January 2021 5,343 7 View

How to download future precipitation data for years 2021-2030?

For my future discharge forecast in the calibrated HEC HMS model, i need the precipitation data of the future.How can i get this data in the daily timescale?

21 December 2020 8,928 5 View

How to perform snowmelt runoff modeling in hEC HMS?

My study area of rainfall-runoff modelling is in mountainous region and has snow cover. There is about 10 percent snowmelt contribution to the discharge of the river i am modelling. How can I...

23 November 2020 7,873 4 View

How to accurately quantify stormwater runoff taking into account both land surface cover and climatic conditions?

I would like to estimate stormwater runoff using a methodology that takes into account both land surface cover and climatic conditions. Is there a reliable approach to achieve that?

18 October 2020 9,325 2 View

How to interpret MSWEP 3-hourly rainfall data 0.1 degree resolution?

I have downloaded the MSWEP sub-daily rainfall data (3-hourly data 0.1 degree resolution). I used MATLAB to convert it and obtained a table of dimensions 1800 x 3600 for a specific date. I am...

18 October 2020 4,205 4 View

Does SWAT model not support high resolution gridded climate (0.25 degree) data?

Dear all, I have prepared rainfall datasets for SWAT MODEL using 0.25 degree IMD long term gridded rainfall data, but as I am using it in the model, it is rounding off the co-ordinates...

03 October 2020 421 5 View

What test should I do on SPSS for the data set described below and how do I feed in this data?

I'm trying to run SPSS to establish a relationship between a) level of air pollution in Italy (independent variable) and b) the number of COVID-19 cases in Italy (dependent variable). For my...

13 September 2020 2,340 3 View

Do have recomendations for open source tools/software in the field hydrology/hydrogeology?

I am doing a small research to review the water related open source tools / software (e.g FREEWAT). if you have used/developed/ heard of similar tools,please share it with me ? Thanks in advance

30 July 2020 4,779 17 View

Could anyone suggest me how can i get the hydrologic soil group classification dataset for Ethiopia?

I am working on surface runoff estimation using SCS CN method and am looking for a national hydrologic soil dataset . I would appreciate for any tips.

26 July 2020 311 8 View

What are the different methods of estimating snow melt contribution in runoff?

I want to estimate the runoff due to rainfall but the data I have, contains final runoff (i.e. runoff + snowmelt) so is there any method to calculate the contribution of snowmelt in the final...

16 July 2020 6,415 3 View