How do I assess when to use "match between runs" for LFQ analysis on Maxquant?

Hi Proteomics enthusiasts, I am currently analyzing DNA pulldown data (treated vs non-treated) using Label-free quantification on Maxquant. I am trying to assess if a protein is present in one...

04 August 2020 1,090 6 View

Why am I unable to detect Histone PTMs at their correct molecular weight in nuclear extracts via Western Blotting?

I am trying to detect changes in Histone PTMs with or without treatment in nuclear extracts from cell lysates. I use a Hypotonic buffer (20 mM Tris pH 7.4, 10mM NaCl, 1mM MgCl2, 0.5mM EDTA)...

10 March 2020 2,626 6 View

Has anyone else seen a reversal of solutions during an mRNA extraction?

I am trying to do an mRNA extraction to be used in qPCR. I am using trizol. When I add the chloroform, I should see a clear aqueous solution containing my mRNA at the top and the pink phenol...

10 April 2019 7,821 3 View

Specific primers for marine chytrids/oomycetes?

03 September 2014 8,892 3 View

Is there a classification scheme for rock cavities or caverns with respect to their size and shape?

The range of forms and sizes of cavities in rock is very big. Is there a classification scheme for rock cavities regarding their size and shape?

02 March 2021 4,511 3 View

Is Journal of Agricultural Research a predator?

it is published by Juniper

28 February 2021 354 4 View

Where can I obtain agricultural land use data for Wales, UK?

The Gower peninsula around Swansea is my target area

16 February 2021 7,473 3 View

How to differentiate the economic productivity in agriculture and in manufacturing sector?

Most of the references I found were on the economic productivity in agriculture but very limited application in manufacturing sector. I would appreciate if anyone could share the references on...

15 February 2021 8,342 7 View

What is the ideal value of UACI for image encryption scheme?

UACI - Unified Averaged Changed Intensity between cipher images. Why some papers refer ideal value to be 33.33 and some refer it to be 33.46? Which is the correct value? (In reference to image...

11 February 2021 9,562 2 View

What are the ways to test the knowledge level of agricultural labourer about their day to day work?

what kind of test can I do for testing the level of knowledge about their daily operation?

03 February 2021 7,583 10 View

What are different ways to compare the histogram of cipher images from various encryption schemes?

Is there a metric to find if one histogram of cipher image is better than other? I tried calculating the variance of the image but couldn't get the intended results. Are there ways to compare...

28 January 2021 479 3 View

Who knows some kind of legal scheme for renewable energy certificates (REC, iREC, GO) integration requirements?

Who is a "central body" for renewable energy source certification and process implementation? Are REC, iREC, GO securities or there is another legal scheme? Is it possible to trade them outside...

21 January 2021 4,179 3 View

What do you think about spatially based sustainable agriculture?

I am currently researching sustainable agriculture. I am interested in elaborating the LCA method and spatial mapping analysis. Most of the sustainable agricultural research only looks at 3...

17 January 2021 6,201 6 View

Which LCIA method is most suitable for assessing the impacts of nitrate leaching in groundwater (eutrophication )?

The eutrophication of groundwater by nitrate leaching is major environmental problem in Germany, especially in areas with intensive agriculture and high N-fertilizer inputs. In addition there are...

16 January 2021 5,182 7 View