I am wondering if anyone has had issues with their image J thresholding? I am trying to perform colour deconvolution, but when I convert the image to binary, I am unable adjust the threshold to accurately differentiate cell bodies. As soon as I adjust the scale bar the image ether goes completely white or black, with no accurate thresholding. Has anyone had this issue before? I have tried updating my computer (mac), updating image J, using a previous version of image J, and also trying on a windows PC, to no avail. Any advice would be amazing as it beats me!

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