Hello everyone

Recently we've bought the Anti-Myosin light chain (phospho S20) antibody from abcam (ab2480). Surprisingly, we detect a specific band at 50 kDa (supposed to be 20 kDa). Does anyone have experienced this issue?

Proteins were resolved in SDS-PAGE. Samples were lysed within

Tris pH 7.5 50 mM

Triton X-100 0.1%

EDTA 1 mM

EGTA 1 mM

NaF 50 mM

Sodium glycerophospate 10 mM

Sodium pyrophosphate 5 mM

Sodium orthovanadate 1 mM

And boiled at 95 ªC for 5 min.

Thank you for your anwers.

Carlos