We do not have turbidity in the first day phage isolation, but in the repeat experiment the following days we encounter turbidity and not the same as the first day.
Please advise us as to how we solve this problem?
If you mean plaque turbidity, the result means that the phage is not truly lytic, but is either lysogenic or slows down the growth of infected cells without actually killing them (e.g. ssDNA pahges such as M13). If you incubate the plates for several days, you will get turbid plaques owing to the growth of lysogens or of infected, but not killed cells
Dear Majid,
I didn't undrestand your question. Are you hoping to isolate lytic phages?Turbidity in liquid medium or on agar plates? in which procedure?
In the initial culture (day 1) the phage was lytic against specific host, hence there was a clearance of culture turbidity. Seems like the bacteria developed resistance to phage at later time points (day 2 or 3), hence the turbidity increased.
The other possibility is phage may turned in to lysogenic as Pierre pointed out.
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