Plz give me a solution..
If serum PCR is positive and the patient is not immunocompromised, acute infection is probable. Combine it with serology: VCA-IgG and -IgM reactivity and negative EBNA-1-Antibody.
Hello all. I grew ARPE-19 cells in cell culture and infected them with a virus (Varicella-zoster virus). After the virus reached a high infection rate, I harvested everything in the plate and use...
07 May 2024 259 3 View
Hi follow virologists, I'm new to the virology field and I'm eager to understand how freeze/thaw cycles affect virus culture fluid titers. I've noticed that while some viruses show increased...
18 March 2024 5,174 4 View
Dear virologists; What is the PCR technique used in virology to detect viral nucleic acids? The steps involved. I'd also like to know, since some viruses have a single strand of DNA and RNA, how...
28 February 2024 1,869 10 View
Is it possible that a hybrid virus is generated in a eukaryotic cell by co-infection of different viruses through nonhomologous recombination?
14 September 2023 7,245 3 View
For diagnostics of Avian infectious bronchitis viruses, I use an end-point RT PCR reaction, and before the RT srage I do a primer annealing stage with the target reverse primer (2uM). My queation...
22 August 2023 1,600 1 View
I am looking for a PhD Position in Microbiology, Virology, and Molecular Biology.
26 July 2023 7,550 1 View
Dear Researchers, I need 2 ml of NZ2 strain ORFV virus for my student Ph thesis. Could you send us, buyer pay. My address: Prof.Dr. Mehmet KALE Department of Virology Faculty of Veterinary...
29 May 2023 7,105 6 View
Hi, I have been reading about vectors and strategies used in engineering insect cell lines. PiggyBac being a very promising system, is not supplied with a autonomously replicating transposase...
17 April 2023 5,873 6 View
I have been looking to expand our virology department here for a year since joining my current institution. I have only ever purchased viruses from Carolina Biosciences (bacteriophage) and ATCC...
12 January 2023 4,248 2 View
Im trying to find an oligoprimer set for HA gene amplification of H5N1? I will appreciate if you could point out a primer set for phylohenetic analysis by H5N1 HA gene sequencing.
27 November 2022 9,614 2 View