We have been trying to isolate the giardia cysts from human fecal sample positive for Giardia(7 days old sample), followed by excystation and further culturing. We started with sucrose flotation technique, then axenization with antibiotic cocktail (Erythromycin, Chloramphenicol, Amikacin Tetracycline, Rifampicin), excystation and then added to TYI-S-33 media. But we got yeast in the culture media and could not find any Giardia.
Dear Archana , Befor cultivation Please incubate cysts in 1% HCL overnight.
Thank you Sir,
So this has to be included after the treatment with excystation stage 1 and 2 solution? And is it advisable to add Amphotericin B to prevent the fungal contamination?
I have used an apparatus called the FlukeFinder < URL: http://www.flukefinder.com/ > to isolate fluke eggs from deer pellet samples. However, it is adaptable for concentrating Giardia cysts.
It involves centrifugation. I don't know if this affects the excystation, but it may be worth trying.
Trevor
Dear Archana,
By confirming Dr.Hooshyar suggestion to incubate cysts in 1% Hcl overnight,adding Amphotericin B will be no problem for growth of parasite and excystation.
Good Luke
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