Is Zymolase or Lyticase better for enzymatic lysis of Saccharomyces cerevisiae cells?

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Murugappan Sathappa

Hey Gaurav,

Based on my experience of crude mitochondrial prep we use Zymolase 3mg/g of S.cerevisiae cells. They are effective in digesting the cell wall and forming spheroplasts.

http://www.amsbio.com/showroom/Zymolyase_Comparison.pdf

This might help you out.

Arvind Panday

Zymolase has greater activity, so with less amount of zymolase and short incubation period, you can get good yield...