Gaurav Chhetri

6 Questions 48 Answers 0 Followers

Questions related from Gaurav Chhetri

Commercially available pre-stained native PAGE marker/ladder??

I'm searching for a commercially available pre-stained native PAGE marker compatible with both native PAGE and Western blotting. The NativeMark™ Unstained Protein Standard works well for native...

25 October 2024 5,707 0 View

Problem regarding 3 way ligation?

Hello everybody!!! I am facing a problem regarding 3 way ligation. I am trying to ligate 2 inserts into a vector with desired gene. Every time after ligation and colonies screening i am getting...

27 September 2016 7,946 3 View

Problem regarding pull down assay. Can anyone lend their expertise?

I am trying to identify interaction partners of my recombinant protein. I set up the pull down assay including my recombinant protein and negative control i.e. having same tag which is present in...

04 July 2014 9,340 3 View

Is Zymolase or Lyticase better for enzymatic lysis of Saccharomyces cerevisiae cells?

I need to extract protein for interaction studies.

26 December 2013 5,929 3 View

Can anyone recommend a method for yeast (Saccharomyces cerevisiae) cell lysis?

Can anyone share the method for yeast cell lysis without using french press, sonication, chemical lysis, or grinding with liquid nitrogen? I need the yeast cell lysate for some protein-protein...

07 December 2013 6,325 27 View

Any advice on rice sample preparation for SDS PAGE analysis?

I want to run an SDS PAGE of rice crude proteins. I used a grinding method with liquid nitrogen, but was unable to make a good sample that I could use for SDS PAGE. Is there any other suggested...

27 November 2013 6,568 5 View