Gaurav Chhetri

4 Questions 44 Answers 0 Followers

Questions related from Gaurav Chhetri

Problem regarding 3 way ligation?

Hello everybody!!! I am facing a problem regarding 3 way ligation. I am trying to ligate 2 inserts into a vector with desired gene. Every time after ligation and colonies screening i am getting...

27 September 2016 7,907 3 View

Problem regarding pull down assay. Can anyone lend their expertise?

I am trying to identify interaction partners of my recombinant protein. I set up the pull down assay including my recombinant protein and negative control i.e. having same tag which is present in...

04 July 2014 9,283 3 View

Can anyone recommend a method for yeast (Saccharomyces cerevisiae) cell lysis?

Can anyone share the method for yeast cell lysis without using french press, sonication, chemical lysis, or grinding with liquid nitrogen? I need the yeast cell lysate for some protein-protein...

07 December 2013 6,278 27 View

Any advice on rice sample preparation for SDS PAGE analysis?

I want to run an SDS PAGE of rice crude proteins. I used a grinding method with liquid nitrogen, but was unable to make a good sample that I could use for SDS PAGE. Is there any other suggested...

27 November 2013 6,507 5 View