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I'm searching for a commercially available pre-stained native PAGE marker compatible with both native PAGE and Western blotting. The NativeMark™ Unstained Protein Standard works well for native...
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Hello everybody!!! I am facing a problem regarding 3 way ligation. I am trying to ligate 2 inserts into a vector with desired gene. Every time after ligation and colonies screening i am getting...
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I have 2 proteins cloned in PET 41a which contains GST tag. To cleave the GST tag I need to digest the expressed recombinant protein with enterokinase. I tried 2-3 times with proper calculated...
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I need to extract protein for interaction studies.
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Can anyone share the method for yeast cell lysis without using french press, sonication, chemical lysis, or grinding with liquid nitrogen? I need the yeast cell lysate for some protein-protein...
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