Murugappan Sathappa

3 Questions 12 Answers 0 Followers

Questions related from Murugappan Sathappa

What am I doing wrong with my phospholipase a2 digestion?

I am trying to use phospholipase a2 (from honey bee venom) to digest bound phospholipids of purified complex in detergent micelles (DDM). I tried to test the phospholipase a2 activity in liposomes...

08 August 2014 9,531 6 View

Can anyone send me a standard protocol which works for cysteine aminoacylation reaction?

I am trying to charge cysteinyl tRNA with cysteine amino acid and then do a labeling with NBD dye using the thiol reactive group of cysteine. I am not getting any luck with the labeling so far,...

02 January 2014 334 2 View

Why is His tag not binding to Ni, or are there any non-specific interactions?

I am trying to purify my His-tagged subunit of a protein complex using a Ni-NTA agarose column. I am doing a WT control with no His tag on the subunit of protein complex. Based on Western Blot,...

09 October 2013 8,377 26 View