Murugappan Sathappa

2 Questions 9 Answers 0 Followers

Questions related from Murugappan Sathappa

Can anyone send me a standard protocol which works for cysteine aminoacylation reaction?

I am trying to charge cysteinyl tRNA with cysteine amino acid and then do a labeling with NBD dye using the thiol reactive group of cysteine. I am not getting any luck with the labeling so far,...

02 January 2014 307 2 View

Why is His tag not binding to Ni, or are there any non-specific interactions?

I am trying to purify my His-tagged subunit of a protein complex using a Ni-NTA agarose column. I am doing a WT control with no His tag on the subunit of protein complex. Based on Western Blot,...

09 October 2013 8,341 26 View