Is Wheat Germ Agglutinin staining(WGA) a suitable method for detection and quantification of liver fibrosis?

11 March 2020 0 5K Report

Is there any reason which suggest this method can / can't be used for staining collagen in liver?

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Science method

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How to perform Gaussian optimization for a polymeric structure?

I am trying to perform Gaussian optimization of a polymeric structure with 250 atoms. It is made up of only C and H atoms with several aromatic phenyl ring interlinked by C=C. Every time I am...

04 January 2021 9,616 2 View

Can I calculate discharge of streams/ watersheds in GIS without field data?

I am required to do a project where I have to calculate discharge of streams/ watersheds in a high altitude area with extremely treacherous terrain. Will it be possible to achieve this objective...

27 December 2020 9,101 5 View

If one publishes in a journal with impact factor >1.4 and which is not included in NAAS list of journals, will the paper be equally valued by ICAR?

I understand the difference between NAAS score and Impact Factor but is it okay to publish in a non-NAAS, high IF journal? How will it be valued in ICAR interviews and related recruitments?

10 September 2020 7,575 3 View

How can I perform sensitivity analysis using R?

I have below model: X = A*B Where A = a list of values with Lognormal distribution (size = 13) and B = another list of values with Lognormal distribution (size = 13) How can I perform 1st...

05 July 2020 1,963 1 View

We have some proteomics data from 2017 and 2018 for which the raw files are missing, but we have the processed raw data. Is the data publishable?

We are missing some mass-spec raw files from 2017 and 2018 when deposition of raw files were not mandatory. Since most of the journals now require raw file availability through ProteomeXchange,...

14 June 2020 6,064 1 View

Why second stage juveniles are used for DNA extraction from nematode for identification purpose? Can I use female or male for the same purpose?

I am working on rice root knot nematode. For identification purpose mostly second stage juveniles are used. I wonder why females or males are not used commonly for the same purpose? I am using...

26 March 2020 7,419 3 View

Is there any alternative cheaper TaqMan universal master mix available in the market other than Thermo with similar sensitivity?

I am looking for a cheaper TagMan master mix for qPCR. I use FAM labeled primers for gene expression, does anyone have any suggestions

17 March 2020 1,105 2 View

What is the effect of Bejan number on heat transfer applications?

In our case the Bejan number decreases with the increase of mass flow rate or Reynolds number of the multiphase fluids through a packed bed. However, if mass flow rate increases Nusselt number,...

27 December 2019 6,088 3 View

Is range of the induced vector measure of a Riemann-Pettis integrable function relatively compact in X?

A function $f\in X^{[a, b]}$ is said to be Riemann-Pettis integrable if $x^* f$ is Riemann integrable for each $x^*\in X^*$ and if for each interval $I\subset [a, b]$, there is a vector in $x_I\in...

27 December 2019 7,532 9 View

Higher or lower values of local entropy generation rate is desirable for the better heat transport?

Local entropy generation rate (comes from the 2nd law of thermodynamics) should be higher or lower for a heat transfer device for its better heat transport performance?

22 December 2019 5,338 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View