Is Wheat Germ Agglutinin staining(WGA) a suitable method for detection and quantification of liver fibrosis?

11 March 2020 0 5K Report

Is there any reason which suggest this method can / can't be used for staining collagen in liver?

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Why is there no peak shifting in FTIR-ATR after the adsorption of PS-NH2 onto the NIPAAM/CMC hydrogel materials?

Why is there no peak shift in FTIR-ATR after the amine-modified polystyrene nanoplastics (PS-NH2) adsorption onto the NIPAAM/CMC hydrogel materials? I have repeated the tests, but I have gotten...

09 June 2024 9,007 6 View

Why am I observing a m/z peak at 372 for the ciprofloxacin hydrochloride hydrate spectra in MS?

The antibiotic powder is dissolved in Milli-q water and then filtered using syringe filter for the analysis.

01 June 2024 7,419 0 View

What is the significance of -ve 'b' value or -ve intercept for the linear Langmuir isotherm plot??

What is the significance of the -ve 'b' value or -ve intercept for the linear Langmuir isotherm plot?? I recently plotted some of my experimental data in a Langmuir linear isotherm plot. I have...

18 May 2024 4,576 10 View

In Electrostatic Force Microscopy, how Phase (degree) is related with Capacitance?

In EFM measurement, we get topography and Phase image. From phase image what we can conclude? Let say, with thickness of a nanosheet phase is increasing. What does it mean that with thickness...

08 May 2024 7,590 1 View

How can I get involved in writing a chapter of scientific book in my research area?

Any lead will be helpful

24 April 2024 5,987 3 View

Why does IR spectrum of a compound of mine show a peak more than 100% transmittance??

The instrument provided data of the IR spectrum is in %T vs wave number. But the peak is in the 3600 to 2600 cm-1 shows more than 100% transmittance. What are the probable reasons behind it? How...

21 April 2024 1,745 1 View

HeLa cells have been detaching after heat stress. Can someone please suggest some way to keep the cells adhered during heat stress and recovery?

I have been growing HeLa to 90-100% confluency in 12 well dish, prior to subjecting them to heat stress at 43 degrees Celsius for 1.5hrs in a water bath. Unlike HEK293T, the HeLa cells lose...

16 April 2024 7,328 0 View

How can I measure the zeta potential of hydrogel beads?

Actually, the Zeta potential measurement instrument (Zeta Sizer, DLS method) needs the refractive index and absorbance values of the hydrogel beads. How can I measure these two parameter values...

28 March 2024 6,947 4 View

Can anyone lease provide a stepwise protocol for catalase assay activity in aerobic/gram-positive bacteria?

Catalase assay for bacteria - not screening test with hydrogen peroxide The complete procedure using spectrophotometer

13 January 2024 8,828 1 View

Hi, can anyone suggest anti-ubiquitin antibody that is specific for mono-ubiquitination and does detect not poly-ubiquitination or free Ubiquitin?

In our in-vivo assays, we see a modified band which we know is a mono-ubiquitin band. We still want to confirm with a mono-ubiquitin specific antibody, if the same is available commercially.

19 November 2023 7,374 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

Can the limit of quantification (LOQ) of an analytical method fall outside its linear dynamic range, or must it always be within it?

Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...

29 July 2024 7,198 9 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View