Does PFA transcardial perfusion artefactually stretch the rodent heart?

Hi everyone I'm comparing aorta/ventricle wall thickness of mouse. Perfusion was done to minimise auto fluorescence during confocal IF. Question is whether transcardial perfusion artefactually...

28 October 2020 8,059 2 View

What emotional safety protocols can be put in place as "first aids" to deal with our anxieties and fears owing to Covid-19?

A lot of physical safety protocols have been upheld all around the globe since the Covid-19 pandemic. What i do not hear often is how to deal with the emotional and psychological distress that...

26 August 2020 1,516 32 View

Which is better in determining the effectiveness of artificial licks for wildlife detection in forested areas?

Hello, I'm planning for my future research study on artificial salt lick. The study site that I've selected for this study has been confirmed with no presence of natural salt lick, thus I plan to...

02 August 2020 5,609 1 View

What statistical tests can I perform on glycomics data collected from disease cohort across 3 timepoints?

I have a dataset comprising clinical characteristics and glycomics profiles of disease samples (n=100+) and matched healthy controls. So far, I have been able to do classification modeling to...

20 July 2020 2,153 3 View

0.5M EDTA buffer preparation: Why does it turn into milky white foam-like mixture?

I am making 200ml of 0.5M EDTA, pH8. I mixed 29.22g EDTA (MW: 292.24g/mol) with 100ml distilled water first (planning to add up to 200ml after measuring pH). I was adding NaOH pellet into the...

03 June 2020 4,581 3 View

How to grow SnO2 nanorods on FTO substrate using hydrothermal method?

i try to immerse SnO2 thin film cotaed-FTO substrate on aqueous solutions (DI water, ethanol and small amount of HCl ), and the experiment is carried out inside lab oven with the temperature of 90...

12 January 2020 4,419 4 View

Which is the best instrument to measure social competence in 12-year-old children?

i am having difficulties in choosing the one and best suitable instrument of social competence for the 12-year-old children. i am planning to combine different questionnaires to measure it, but...

11 December 2019 1,591 3 View

Apart from western blot, what are the other tests that can be used to determine expression of autophagy-related proteins ?

I would like to know if there are other options that requires less cost.

09 July 2019 6,974 2 View

Social competence scale for grandparents-raising grandchildren?

Hi, I am Hoon. I am currently working on my master thesis on grandparenting and its influences on child's social competence. i failed to find suitable child social competence scale for 12-year-old...

28 March 2019 8,832 3 View

How do I minimize exposure to room air just after exposure to hypoxia?

I am planning an lab study on effect of hypoxia on glucose metabolism of cancer cell. For hypoxia exposure, I am considering anaerobic chamber system. My concern is that if I use this chamber,...

14 March 2019 7,641 8 View

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03 March 2021 5,360 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

Online surveys for social sciences researchs: what are your opinions about this tool and how can we improve the research design to incorporate online?

Good morning everyone, Due to the pandemic, I conducted several online surveys. It is quite agile, not at all expensive, but there are several problems: the sample's representativeness and its...

21 February 2021 7,457 3 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

ZnS oxidizes to ZnO!!why??

when I put my ZnS solution in the chemical bath, the ZnS oxidizes so there are ZnO peaks appearing because of the reaction with the oxygen in the air, despite the fact that I close the beaker...

14 February 2021 5,778 9 View

Hi, can a variable in a questionnaire be measured with a series of questions or it has to one question for one variable?

I am doing research on the impact of competitive bidding on project success and would like to use SEM. Project success shall be: cost overage, completion time, quality of works and variation...

13 February 2021 4,413 6 View

Why non-specific band appear on T-ARMS PCR?

Hi everyone. We want to know how can I remove the non-specific band amplification on this variant. Although the amplification of non-specific band is not as intense as the band that we want, but...

09 February 2021 588 2 View

What is your opinion regarding opening of school? Now students are supposed to join school. Do you think that decision is in the interest of society ?

From long Pendamic now government is opening school and colleges on for off line classes. What do you think that this decision is fruitful to the students and society too?

08 February 2021 3,068 6 View

Fractional derivative users, are you on the same page with me on the validity of the fractional order parameter, say alpha?

Taking a fractional ODE problem as an example. It has come to my attention that if the fractional order parameter is in the interval (0,1], many only presents results for alpha=0.9, 0.99. I saw...

08 February 2021 9,671 3 View