Is this typical lymphocyte, the flower cells?

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Generating multiple standard curves from a single template?

Hi everyone! After thinking about it on my own, I'm posting a question here. Has anyone ever tried creating qPCR standard curve samples (plasmid or DNA standard) by inserting multiple target...

30 June 2024 1,569 1 View

How to purify boronate esters?

How to purify boron esters? Hello. I'm synthesizing borates in my lab. My questions are as follows: 1. How to purify boronate esters like B(OR)3 compounds, and it is a UV undetected...

23 May 2024 7,072 2 View

Apart from the stadiometer, what is the next best measure for height among 6-10 year old children?

We are currently doing community work where we have to measure the height and weight of children 6-10 years old. The community work is done across different communities. Thus, since the...

03 April 2024 7,311 0 View

RNA QC for RNASeq - Proceed or not proceed?

Hi All, I would like to seek for your expert input for below queries on RNA QC for RNASeq: Can we proceed for RNASeq run on RNA less than 10ng, with DV200 in between 68 - 80%?Details as below:...

18 March 2024 5,936 2 View

Why is there no visible opalescent band formed after sucrose density gradient ultracentrifugation for phage purification?

I am currently conducting research on purifying Myoviridae phage particles using sucrose density gradient ultracentrifugation. However, I have encountered a challenge where no visible opalescent...

12 March 2024 9,387 3 View

What is the reason for %RSE=0 ?

hi I just start building a nonmem pharmacokinetics model. i got a problem with my estimate result .. is there any reason for %RSE=0?? if anyone know about this issue , please explain this...

04 March 2024 6,077 0 View

Does fixing cells kill intracellular proteins?

I'm following a protocol to bind fluorescent ligand to receptors expressed on cells in a microplate. The protocol includes fixing cells with 4% PFA for 20 mins. This prevents cell loss during...

09 January 2024 7,517 7 View

Cross Reference by DOI issued by ResearchGate?

Looking for somebody who had similar experience when you use DOI issued by ResearchGate. I published one paper in a conference in 2007. The conference seems not to publish their proceeding, and I...

25 December 2023 5,537 1 View

Social cognitive - the empathy of brain development and its effect ?

Required a research paper with raw data to conduct a comparison of recent research and future direction

05 December 2023 2,528 1 View

How to obtain a compact film using atomic layer deposition method?

Could I obtain a compact film through the atomic layer deposition method? If yes, why as-deposited SnO2 film with a thickness of 17 nm fail to protect the underneath layer film of my device? Could...

05 November 2023 8,153 0 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Request for Advice: Starch Metabolism Research Project?

I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...

30 July 2024 4,267 2 View

What are the important scholarly articles related to 'Use of Likert Scale in social science research' ?

The Likert scale is a commonly used measurement tool in social science research to assess people's attitudes, opinions, or perceptions. The social science students still have difficulty selecting...

25 July 2024 7,535 1 View

Is it fare for editors of reputed Journals to put on hold manuscripts more than six months without review updates, Are they harassing researchers?

It is being seen that Editors of some reputed Journals are putting on hold the manuscripts and not updating review states even passing more than six months from submission. Researchers are...

24 July 2024 8,596 2 View

How to transfect circular mRNA to T cells?

Hello! We are using LNP to deliver circular mRNA into T cells. However, compared with linear mRNA, the transfection efficiency and protein expression duration are similar. Are LNP generation or...

21 July 2024 4,322 1 View

How to perform immunofluorescence on suspension cells?

Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...

20 July 2024 3,797 2 View

China (Qinghai) flowers identification?

Can someone help me in identification of some flowers observed in Tibetan plateau (Qinghai province)? See the attached pdf. Sincerely Armando

18 July 2024 5,125 4 View

How to activate 4E8 T cells with plate-bound anti-CD3 for a genome-wide CRISPR screen?

It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...

17 July 2024 3,371 0 View

How to culture and stimulating hamster splenocytes for T-cell analysis and ELISPOT?

Hi, Does anyone have experience and success of isolating, culturing and stimulating hamster splenocytes for T-cell analysis (FACS and ELISPOT)? I am currently using a protocol that we routinely...

09 July 2024 6,336 1 View

How to maintain intestinal tissue resident cells (TRM) in culture?

I am attempting to culture tissue resident T cells (TRM) derived from fresh intestinal tissue for up to six days. I am finding that the viability of the T cells is low at D0, and a very small...

08 July 2024 4,887 2 View