How do I choose protein targets in breast cancer cell lines inorder to carry out insilico studies of phytochemical components against MCF-7?

Hello, Please suggest me how to select protein targets in MCF-7 for molecular docking studies of phytoconstituents against breast cancer cell lines. I have seen in research papers regarding the in...

03 April 2016 3,265 6 View

What is the importance of using frosted slide while doing comet assay?At how much thickness, NMPA is to be layered on slide?

I am presently doing come assay. But, I don't get results.

31 December 2014 265 3 View

Please suggest the relevance of using Normal Melting Point Agar and Low Melting Point Agar while doing comet assay?

Can we use NMPA (Normal Melting Point Agar) instead of LMPA?

10 November 2014 6,691 9 View

Which primers are commonly used for the RAPD analysis of marantaceae (Maranta arundinacea)?

Can the primers used for the RAPD analysis of zingiberaceae be used for the family marantaceae?

09 October 2014 8,751 3 View

Can antigenotoxicity of plant extract be discussed in which terms other than reduction in Mitotic Index?

07 August 2014 5,276 5 View

What precautions need to be taken while doing the in-vitro propagation of a laticiferous plant?

07 August 2014 5,005 10 View

Is there any effect of plant extract on cell wall breakage?

06 July 2014 1,241 10 View

Can anyone suggest a tip to avoid cytoplasmic staining?

For observing chromosome aberration, treated allium cepa root tips were stained with Acetocarmine and destained with 45% acetic acid. But while obseving under microscope, cytoplasm is also...

05 June 2014 1,934 2 View

Is there any preliminary screening assay for the production of aflatoxin by Aspergillus flavus?

Seeking an answer.

05 June 2014 7,565 5 View

Is there any specific protocol for the extraction of DNA in Sarcostemma plant (apocynaceae)?

Part of my research.

05 June 2014 1,651 5 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View