I am screening antigenotoxicity potential of a plant on Allium Cepa root meristems treated with Hydrogen peroxide.
Hi, Neethu, can you explain your question more precisely? Generally, mitotic index may serve as marker of plant extract protection. Ea. you can pre-tretae allium cepa roots with different plant extract and treteda thereafter with H2O2. If you have higher mitotic index after pre-tretamenht and tretament tehreafter, it may mena that given extract at give concehtration have some protective effect.
Cheers!
Hello Taras,
Thank you for the reply. How can I present the anti-genotoxic effect of the plant extract? Can it be explained only in terms of the reduction in MI?
To assess antigenotoxic effect, you can check if the extract is reducing or inhibiting chromosomal damage or micronucleus induced by hydrogen peroxide. You will compare the frequency of chomosomal damage or micronucleus in the hydrogen peroxide treated group and the hydrogen peroxide + extract treated group to see if there is a reduction with the extract treatment
It is also a very good idea to study effect of plant extract on chormosome damage or micronucleus. But for this it is better to use suspension cell: in the roots nucleus population is too heterogenous and have a different sensitivity to h2O2 dependent form cell file, cell position etc.
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