Can anyone suggest a tip to avoid cytoplasmic staining?

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How do I choose protein targets in breast cancer cell lines inorder to carry out insilico studies of phytochemical components against MCF-7?

Hello, Please suggest me how to select protein targets in MCF-7 for molecular docking studies of phytoconstituents against breast cancer cell lines. I have seen in research papers regarding the in...

03 April 2016 3,344 6 View

What is the importance of using frosted slide while doing comet assay?At how much thickness, NMPA is to be layered on slide?

I am presently doing come assay. But, I don't get results.

31 December 2014 361 3 View

Please suggest the relevance of using Normal Melting Point Agar and Low Melting Point Agar while doing comet assay?

Can we use NMPA (Normal Melting Point Agar) instead of LMPA?

10 November 2014 6,788 9 View

Which primers are commonly used for the RAPD analysis of marantaceae (Maranta arundinacea)?

Can the primers used for the RAPD analysis of zingiberaceae be used for the family marantaceae?

09 October 2014 8,837 3 View

Can antigenotoxicity of plant extract be discussed in which terms other than reduction in Mitotic Index?

I am screening antigenotoxicity potential of a plant on Allium Cepa root meristems treated with Hydrogen peroxide.

07 August 2014 5,371 5 View

What precautions need to be taken while doing the in-vitro propagation of a laticiferous plant?

The plant which I am planning to do in-vitro propagation is Sarcostemma. Can I use its internodal and nodal part as explant material? Does its latex cause any contamination or browning of medium?

07 August 2014 5,183 10 View

Is there any effect of plant extract on cell wall breakage?

While doing cytotoxicity studies of Cyanchum on Allium cepa root meristem, majority of cells were found to be broken. Is there any effect of the biomolecule on it? Which of the phytocompounds (in...

06 July 2014 1,361 10 View

Is this bacteria Proteus?

Gram staining. Can you confirm this bacteria is Proteus from the image attached?

05 June 2014 8,499 5 View

Is there any preliminary screening assay for the production of aflatoxin by Aspergillus flavus?

Seeking an answer.

05 June 2014 7,652 5 View

Is there any specific protocol for the extraction of DNA in Sarcostemma plant (apocynaceae)?

Part of my research.

05 June 2014 1,746 5 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

While using the IHC PROFILER plugin in image j software, to quantify the epithelial cytoplasmic DAB staining do you crop remove the stromal tissue?

My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...

29 July 2024 2,682 6 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

How to restart MD without using checkpint file in GROMACS?

Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar

29 July 2024 3,084 8 View