Primary screening can be done by agar diffusion method. It will give you qualitative results. Plate out the bacterial culture sensitive to Afla toxin on a Nutrient Agar plate and make a cup in the center of the plate. Add the spore suspension of the potential Asp flavus culture in the cup and incubate at RT for 3 to 4 days. If the fungal culture liberates the toxin, it will diffuse through the medium and will inhibit the bacterial culture, giving a zone of inhibition, indicating positive results.
Primary screening can be done by agar diffusion method. It will give you qualitative results. Plate out the bacterial culture sensitive to Afla toxin on a Nutrient Agar plate and make a cup in the center of the plate. Add the spore suspension of the potential Asp flavus culture in the cup and incubate at RT for 3 to 4 days. If the fungal culture liberates the toxin, it will diffuse through the medium and will inhibit the bacterial culture, giving a zone of inhibition, indicating positive results.
Hi,
I agree with the above mentioned answer and beside that you can also try basic TLC analysis to detect the production of toxin during its growth and development.
Dear Neethu,
Commercially available aflatoxin test strips are probably the best, easiest and time saving way for preliminary screening assay of aflatoxin.
As a ready reference I am sharing the kit info and the protocol.
Hope that helps.
- Badges
- Science topic
- Similar topics
- Biological Science
- Bioscience
- Biotechnology