While doing cytotoxicity studies of Cyanchum on Allium cepa root meristem, majority of cells were found to be broken. Is there any effect of the biomolecule on it? Which of the phytocompounds (in general) are responsible for cell breakage?
Neethu -
Plant secondary metabolites, for example flavonoids and polyphenolic molecules bind and inhibit specific proteins, and disrupt the cell membrane; they also intercalate into bases of DNA or RNA. I am not sure of their effects on cells walls. I am guessing that you are studying the cytotoxicity of a vine on Allium cepa root meristem cells.
Thank You Mr. Chinthan ...Cynanchum is a xerophytic creeping herb belongs to Apocynaceae
Thanks, Neethu.
On another issue, does does this herb synthesize the flavone molecule, apocyanin? Thanks.
Neethu, you got me! I thought that I made a typographical error. The twp terms appear to be interchangeably employed. In most cases, it appears to be apocynin in articles, which sort of makes sense as it is generally found in Apocynaceae. Thanks...
Hello Morris,
Cells were appeared to be disentegrated by vacoulation.
Cell breakage describes the phenomenon of cell disruption. In mammalian cells with plasma membrane containing phospholipid bilayers with the hydrophobic lipids in the membrane partitioning outside it would be lysis. Plant cells have very thick walls with chlorophyll and other carbohydrates, and cannot be easily lysed.
Hi,
Yes this phenomenum has been observed in animals mainly insects. Please browse the papers in "Pesticide Biochemistry and Physiology" and "Micron" both from elsevier.
Regards,
Do you mean disruption owing to hydrolases from the lysosomes? Lysosomes are known, I guess, in root meristem and epidermis of Allium cepa. Lysosomal disruption (and effect on lipids) is likely. You are determining cytotoxicity; the herb sample might be releasing enzymes. If you are using an extract of your herb the chances are that membrane disruption occurs. Binding and inhibiting proteins in the cell wall might cause disruption at the point of contact.
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