Does cardiac puncture damage the heart for protein analysis or histological staining?

Hello, we have been sacrificing mice and rats using cardiac puncture. I am curious if anyone knows of publications or has had personal experience relating to potentially altered protein...

31 July 2020 681 2 View

Survey for a scientific research in transportation engineering please help?

11 March 2020 4,167 7 View

Is sonicating cells for western blot necessary?

Once cells have been collected in RIPA, before centrifugation (16,000g for 20 min) should cells be sonicated? Does sonication improve protein yield?

14 May 2019 2,385 7 View

What is the purpose of heating RNA at 55-60 C when utilizing the trizol protocol during the last step when RNA is in water?

Does heating influence RNA quality? My understanding was the RNA should not be heated and kept on ice. Trizol protocol:...

13 January 2019 7,933 4 View

Does protein concentration change between freeze and rethaw for western blot??

Hello, My understanding is that protein from cell and tissue lysates degrade between each rethaw. Has anyone experienced differing protein concentrations, significant enough to affect loading...

09 December 2018 6,962 4 View

Hi. Does anybody know an easy way to build the complete sequence of a gene from RNAseq data?

I am trying to identify the unknown sequence of an gene from a plant species which has published RNAseq data deposited to SAR-NCBI. As RNA seq data are all short read data, i am looking for an...

24 February 2018 1,238 2 View

Hi. Does anybody know an easy way to build the complete sequence of a gene from RNAseq data?

I am trying to identify the unknown sequence of an gene from a plant species which has published RNAseq data deposited to SAR-NCBI. As RNA seq data are all short read data, i am looking for an...

01 February 2018 4,649 2 View

How I can relate the loading frequency to the operational vehicle speed in the dynamic modulus of elasticity of asphalt pavement?

28 February 2017 7,686 2 View

How can I optimize the procedure to determine platelet CD markers expression by flow cytometry?

Greetings, Could you please clarify these points to me. I have a study on platelet CD markers expression by flowcytometrey: -If I prepared platelet rich plasma without fixing the cell # and...

12 December 2015 8,820 13 View

How can I optimize the procedure to determine platelet CD markers expression by flow cytometry?

Greetings, Could you please clarify these points to me. I have a study on platelet CD markers expression by flowcytometrey: -If I prepared platelet rich plasma without fixing the cell # and...

11 December 2015 9,870 14 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View