Does cardiac puncture damage the heart for protein analysis or histological staining?

Hello, we have been sacrificing mice and rats using cardiac puncture. I am curious if anyone knows of publications or has had personal experience relating to potentially altered protein...

31 July 2020 681 2 View

Is sonicating cells for western blot necessary?

Once cells have been collected in RIPA, before centrifugation (16,000g for 20 min) should cells be sonicated? Does sonication improve protein yield?

14 May 2019 2,385 7 View

What is the purpose of heating RNA at 55-60 C when utilizing the trizol protocol during the last step when RNA is in water?

Does heating influence RNA quality? My understanding was the RNA should not be heated and kept on ice. Trizol protocol:...

13 January 2019 7,933 4 View

Does protein concentration change between freeze and rethaw for western blot??

Hello, My understanding is that protein from cell and tissue lysates degrade between each rethaw. Has anyone experienced differing protein concentrations, significant enough to affect loading...

09 December 2018 6,962 4 View

Hi. Does anybody know an easy way to build the complete sequence of a gene from RNAseq data?

I am trying to identify the unknown sequence of an gene from a plant species which has published RNAseq data deposited to SAR-NCBI. As RNA seq data are all short read data, i am looking for an...

24 February 2018 1,238 2 View

Hi. Does anybody know an easy way to build the complete sequence of a gene from RNAseq data?

I am trying to identify the unknown sequence of an gene from a plant species which has published RNAseq data deposited to SAR-NCBI. As RNA seq data are all short read data, i am looking for an...

01 February 2018 4,649 2 View

How I can relate the loading frequency to the operational vehicle speed in the dynamic modulus of elasticity of asphalt pavement?

28 February 2017 7,686 2 View

Is there tables to get trip generation rates for marine yard in Gulf area?

12 April 2016 980 2 View

How can I optimize the procedure to determine platelet CD markers expression by flow cytometry?

Greetings, Could you please clarify these points to me. I have a study on platelet CD markers expression by flowcytometrey: -If I prepared platelet rich plasma without fixing the cell # and...

12 December 2015 8,820 13 View

How can I optimize the procedure to determine platelet CD markers expression by flow cytometry?

Greetings, Could you please clarify these points to me. I have a study on platelet CD markers expression by flowcytometrey: -If I prepared platelet rich plasma without fixing the cell # and...

11 December 2015 9,870 14 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

How to find the mean, SD and CI in SPSS?

Hi, I hope that someone can help me with this task: I have 5 groups of participants which are numbered in SPSS from 1 to 5 (according to their group number) and are all under the variable group....

03 March 2021 506 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

No title

02 March 2021 3,060 3 View

No title

02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View