Is there someone who can share their protocol for immunostaining for cells cultured on polyacrylamide gels?

14 April 2021 1 4K Report

I'm having trouble with a nuclear protein immunostain of cells (lung fibroblasts) cultured on soft (1 kPa) collagen-coated hydrogels. Can anyone give me a suggestion?

Alexandr Chernov

Hello, Fernanda!

Please You look at the following articles:

https://jcs.biologists.org/content/joces/131/10/jcs209932.full.pdf

IMR-90 cells (ATCC), from a frozen stock

Immunocytochemistry

Article A practical guide to hydrogels for cell culture

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