I'm using RNAprep Pure Kit for purification of total RNA from plant cells and tissues, My samples are from potato leaves
Validation of purity and quantity of gRNA in your samples are best done using spectrophotometry methods(e.g Nanodrop)..I suppose the DNAse I treatment should take place before the elution of your RNA samples from the columns as this is crucial to its purity.This question will be better answered if you mention why the DNAse I treatment was excluded for some and included for others.
you might have sheared mRNA and however for best RNA QC you should run Qiaxcel or Bioanalyzer and get RNA integrity number between 7 to 9 this is ratio b/w 18s/28s ...looing to your gel... ur purufucation has not gone that well... again if your plan is for rtpcr try to make cDNA and try PCr you might get results.. hwoever for NGS this is not good... and joke..nanodrop might not give you quality but quantity... we need RIS or RIN values
Yes I want to do cDNA synthesis , can I use them? I think the quality ain't good enough
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