Working with insect primary cells in cell medium and in saline solution?

I work with insect primary cells, which I let grow in TC100 insect medium. For fluorescence measurements I have to discard medium (because of its fluorescence properties) and give a saline...

28 January 2021 3,867 1 View

Does anybody work with CoolLED in fluorescence microscopy?

I have trouble with exciting wavelengths higher than 460 nm while e.g. ex with 405 nm works. Filtercubes are correc, as I used them with my old xenon lamp before... I work on axiovert200M and with...

25 January 2021 5,201 2 View

Myeloperoxidase Colorimetric Activity Assay Kit (Sigma-Aldrich, ELISA MAK068). When do we stop the reaction?

We'd like to use this kit for brain tissue samples (rat). When do we stop the reaction, 30, 60, and 120 minutes? We don't like to test at all three stop points, because we've too many samples.

17 January 2021 6,744 2 View

Quantitative research - hypothesis formulation necessary?

Dear all, I am planning to undertake quantitative research with a survey for my thesis. I am wondering if the hypothesis formulation is compulsory or am I able to do my research without it? My...

29 July 2020 6,145 5 View

Does anybody know if there exist research about a possible impact from military used electromagnetic waves frequencies on insects or VLF-waves?

I am very interested in the possible impact of radiation, espacially electromagnetic waves on insects. I searched several times in different online libraries, if there exists any research about...

29 April 2020 5,918 2 View

Could the new starlink satellites be dangereous for the insects due to the electromagnetic waves which they sent to the surface?

There are now many new satellites and are becoming very more ( 30 000) in the next future. I read in the internet that this satellites have not ready investigates risks on healthy and nature due...

23 April 2020 3,660 5 View

Why do I get different protein concentrations by homogenizing?

I implemented a method for protein extraction of skin samples. I did not change the procedure but over time the protein yield decreased. I homogenized skin biopsies using a bead mill homogenizer...

16 April 2020 3,170 7 View

Can anyone recommend a database for dynamic facial expressions that also include expressions of complex emotions?

Most of the databases of dynamic stimuli for facial expressions only include faces expressing basic emotions. Is anyone aware of a database that also includes dynamic face expression stimuli on...

05 November 2019 8,263 2 View

Approved abbreviations for common rat strains?

Hello, I would like to ask if anyone is aware of an existence of an approved abbreviations for common RAT strains. I know that such list is available for the MICE inbred strains, and would like...

08 May 2019 8,103 1 View

Where to publish in the field of cultural management/ academic galleries and museums/ interdisciplinary cooperation/ international networking?

Dear all, I am at the beginning of my academic career and I would like to ask you for your help and experiences? I am interested into peer- reviewed journals with impact (at least Web of...

05 April 2019 9,457 3 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

Why cannot world and community leaders be gentle? Where did ethics of care go?

The whole world is in a mess and paused. We all are tired of seeing hypermasculinized and destructive leaderships all around us. This world has seen enough of the aggressive patriarchal social...

23 February 2021 292 7 View

What is the most prevalent drug related problem in the developed countries?

Detecting the drug related problems is an important step in the pharmaceutical care plan

21 February 2021 5,421 3 View

Can I simply dilute 25% NH3 solution to 10% solution by pouring water in tank and circulating with pump?

Is there any heat of dilution which I need to take care by putting some circulation cooler? Will there be any significant ammonia emission in the process?

09 February 2021 5,943 2 View

Quality of antibodies from Creative Biolabs?

It seems Creative Biolabs has everything and so many versions of antibodies not owned or sold by any other company. How reliable are their products? I am looking for a clone where potentially...

08 February 2021 2,167 3 View

Why marketers need a forensic understanding of consumers and a creative, agile mindset?

02 February 2021 6,579 10 View

When is the right time to stop training a classification algorithm?

In this example giving 400 samples looks like there is an accuracy of 91.5% while training beyond that point, accuracy is getting decreased. Are 400 samples enough to keep for this example? If...

27 January 2021 5,657 3 View

What contributions could be done in the medical emergency field based on voice recognition and NLP?

Hello, My research work focuses on the use of NLP and voice recognition for the medical emergency assistance. So I would appreciate it if you suggest me some contributions that could be done in...

27 January 2021 2,899 3 View

What are the IT and Artificial intelligence contributions to assisting medical emergency cases?

Hello, I am seeking recommendations from the Research Gate community regarding studies about the IT and Artificial intelligence solutions to assisting medical emergency cases. What are the...

23 January 2021 2,667 21 View

I am looking to develop a basic oral microbiome assay. Can anyone advise on culture vessel, conditions and organisms that require minimal maintenance?

I am looking to investigate oral care products with an easy-to-prepare culture of orally derrived microorganisms.

21 January 2021 3,448 1 View