Please explain this Correlation diagram?

Hi Would you mind please explain this correlation diagram between gene Y and X expression. What is R Sq Linear? What is rs? Thank you

05 June 2019 8,383 5 View

SiRNA Transfection: effects of Time and Dose?

I have a question about transfection method with siRNA and transfection reagent. How can we explain the best transfection dose with 60 pmol among different doses (20, 40, 60, 80)? Why did not 80...

11 December 2018 1,523 4 View

Suggest a journal with molecular oncology scope with IF more than 5 and free publishing & free open access?

Hi please suggest a journal with molecular oncology scope with IF more than 5 and free publishing & free open access. Thank You

09 October 2018 4,822 2 View

Can we use controllers of other articles in ours?

Hi I have a question.We did a clinical research for measuring a specific CD marker in special patients. But we forgot to measure it in normal people. Can we use the measurements of this marker as...

07 August 2018 6,275 3 View

Which Drugs is HL60 resistant?

I'm looking for drugs that HL60 can tolerate and is resistant to them. Do you know some of them? Please answer with reference :)

05 June 2018 4,191 2 View

What are other techniques than flowcytometery for detecting Cancer Stem Cell (CSC) properties ?

I know that by flowcytometery and RT-PCR, CSC markers and their expression can be detected? Do you know other techniques for detecting CSCs and its change during drugs treatments?

05 June 2018 2,018 10 View

How to reference an In press article?

Hi, I have an article which is in press and have DOI but, it has not yet been published. How can I reference to this article in another article?

01 January 1970 5,843 8 View

The best book for English editing ??

Hi, I am looking for a book that will teach you how to edit English texts. What do you think is the best book in this field?

01 January 1970 8,610 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View